Supplementary Materials

Supplementary Material for:

Identifying DNA methylation biomarkers for non-endoscopic detection of Barrett's esophagus

Helen R. Moinova, Thomas LaFramboise, James D. Lutterbaugh, Apoorva Krishna Chandar, John Dumot, Ashley Faulx, Wendy Brock, Omar De la Cruz Cabrera, Kishore Guda, Jill S. Barnholtz-Sloan, Prasad G. Iyer, Marcia I. Canto, Jean S. Wang, Nicholas J. Shaheen, Prashanti N. Thota, Joseph E. Willis,* Amitabh Chak,* Sanford D. Markowitz*

*Corresponding author. Email: SXM10{at}cwru.edu (S.D.M.); josephe.willis{at}uhhospitals.org (J.E.W.); amitabh.chak{at}uhhospitals.org (A.C.)

Published 17 January 2018, Sci. Transl. Med. 10, eaao5848 (2018)
DOI: 10.1126/scitranslmed.aao5848

This PDF file includes:

  • Fig. S1. Flowchart of study analyses.
  • Fig. S2. Comparison of methylation of individual CpGs versus CpG patches in discriminating esophageal lesions.
  • Fig. S3. Comparing VIM and CCNA1 expression versus methylation.
  • Fig. S4. Morphology of touch preps from balloon brushings of three intact porcine esophagus samples from esophagogastric organ explants.
  • Table S1. Location of 26 differentially methylated patches identified by RRBS comparison of normal esophageal squamous mucosa versus Barrett’s lesions.
  • Table S2. Demographic characteristics of training and validation esophageal brushing populations.
  • Table S3. mVIM and mCCNA1 performance in training and validation esophageal brushing samples.
  • Table S4. Comparison of sensitivities of mVIM plus mCCNA1 versus mVIM or mCCNA1, all at equal specificities.
  • Table S5. Influence of smoking on VIM and CCNA1 methylation in proximal versus distal esophagus.
  • Table S6. Participant evaluation of the non-endoscopic balloon sampling of the esophagus.
  • Table S7. Demographic characteristics of subjects in non-endoscopic balloon study.
  • Table S8. Methylation in post-ablation subjects.
  • Table S9. Post-examination questionnaire.
  • Table S10. Bisulfite-specific methylation-independent PCR primer sequences.
  • Table S11. Index tags for bisulfite-specific methylation-independent PCR primer sequences.
  • References (3366)

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