Supplementary Material for:

Inhalation of peptide-loaded nanoparticles improves heart failure

Michele Miragoli,* Paola Ceriotti, Michele Iafisco, Marco Vacchiano, Nicolò Salvarani, Alessio Alogna, Pierluigi Carullo, Gloria Belén Ramirez-Rodríguez, Tatiana Patrício, Lorenzo Degli Esposti, Francesca Rossi, Francesca Ravanetti, Silvana Pinelli, Rossella Alinovi, Marco Erreni, Stefano Rossi, Gianluigi Condorelli, Heiner Post, Anna Tampieri, Daniele Catalucci*

*Corresponding author. Email: michele.miragoli{at}humanitasresearch.it (M.M.); daniele.catalucci{at}cnr.it (D.C.)

Published 17 January 2018, Sci. Transl. Med. 10, eaan6205 (2018)
DOI: 10.1126/scitranslmed.aan6205

This PDF file includes:

• Materials and Methods
• Fig. S1. Size stability of CaPs and CaPs functionalized with MP and HA in protein-free and protein-enriched aqueous medium, and surface charge of CaP-Cy7 and CaPs with Cy7 adsorbed on their surface.
• Fig. S2. In vivo and ex vivo characterization of CaPs.
• Fig. S3. Echocardiographic analysis of CaP-treated mice.
• Fig. S4. Stability, loading, degradation, and structural characterization of CaPs and CaPs functionalized with MP and HA.
• Fig. S5. STED microscopy of isolated cardiomyocytes from mice treated with unloaded CaPs.
• Fig. S6. MP-CaP treatment of diabetic mice.
• Table S1. Echocardiographic analysis of 10-week-old male wild-type mice treated with CaP.
• Table S2. Excitability and refractoriness parameters obtained by epicardial multiple lead recording in untreated rats or rats treated with CaP.
• Table S3. CaP-MP loading.
• Table S4. Echocardiographic analysis of 10-week-old male wild-type and STZ mice treated with MP, CaP-HA, and CaP-MP.
• Table S5. Characterization of LTCC current during rectangular double-pulse protocols and membrane capacities measured in adult cardiomyocytes isolated from treated mice.
• Table S6. Thiobarbituric acid–reactive substance detection in mice treated with MP, CaP-HA, and CaP-MP.
• Legends for movies S1 to S3

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Other Supplementary Material for this manuscript includes the following:

• Movie S1 (.mov format). 3D FMT imaging of the cardiopulmonary area from Cy7-treated mice.
• Movie S2 (.mov format). 3D FMT imaging of the cardiopulmonary area from CaP-Cy7–treated mice.
• Movie S3 (.mov format). STED microscopy of isolated cardiomyocytes from mice treated with MP-rhodamine–loaded CaPs.