Supplementary Materials
Inhalation of peptide-loaded nanoparticles improves heart failure
Michele Miragoli,* Paola Ceriotti, Michele Iafisco, Marco Vacchiano, Nicolò Salvarani, Alessio Alogna, Pierluigi Carullo, Gloria Belén Ramirez-Rodríguez, Tatiana Patrício, Lorenzo Degli Esposti, Francesca Rossi, Francesca Ravanetti, Silvana Pinelli, Rossella Alinovi, Marco Erreni, Stefano Rossi, Gianluigi Condorelli, Heiner Post, Anna Tampieri, Daniele Catalucci*
*Corresponding author. Email: michele.miragoli{at}humanitasresearch.it (M.M.); daniele.catalucci{at}cnr.it (D.C.)
Published 17 January 2018, Sci. Transl. Med. 10, eaan6205 (2018)
DOI: 10.1126/scitranslmed.aan6205
This PDF file includes:
- Materials and Methods
- Fig. S1. Size stability of CaPs and CaPs functionalized with MP and HA in protein-free and protein-enriched aqueous medium, and surface charge of CaP-Cy7 and CaPs with Cy7 adsorbed on their surface.
- Fig. S2. In vivo and ex vivo characterization of CaPs.
- Fig. S3. Echocardiographic analysis of CaP-treated mice.
- Fig. S4. Stability, loading, degradation, and structural characterization of CaPs and CaPs functionalized with MP and HA.
- Fig. S5. STED microscopy of isolated cardiomyocytes from mice treated with unloaded CaPs.
- Fig. S6. MP-CaP treatment of diabetic mice.
- Table S1. Echocardiographic analysis of 10-week-old male wild-type mice treated with CaP.
- Table S2. Excitability and refractoriness parameters obtained by epicardial multiple lead recording in untreated rats or rats treated with CaP.
- Table S3. CaP-MP loading.
- Table S4. Echocardiographic analysis of 10-week-old male wild-type and STZ mice treated with MP, CaP-HA, and CaP-MP.
- Table S5. Characterization of LTCC current during rectangular double-pulse protocols and membrane capacities measured in adult cardiomyocytes isolated from treated mice.
- Table S6. Thiobarbituric acid–reactive substance detection in mice treated with MP, CaP-HA, and CaP-MP.
- Legends for movies S1 to S3
Other Supplementary Material for this manuscript includes the following: