Supplementary Materials

Supplementary Material for:

Methicillin-resistant Staphylococcus aureus causes sustained collecting lymphatic vessel dysfunction

Dennis Jones, Eelco F. J. Meijer, Cedric Blatter, Shan Liao, Ethel R. Pereira, Echoe M. Bouta, Keehoon Jung, Shan Min Chin, Peigen Huang, Lance L. Munn, Benjamin J. Vakoc, Michael Otto, Timothy P. Padera*

*Corresponding author. Email: tpadera{at}steele.mgh.harvard.edu

Published 17 January 2018, Sci. Transl. Med. 10, eaam7964 (2018)
DOI: 10.1126/scitranslmed.aam7964

This PDF file includes:

  • Materials and Methods
  • Fig. S1. MRSA infection leads to acute inflammation and CLV dilation.
  • Fig. S2. CLV function remains impaired after the clearance of MRSA.
  • Fig. S3. Attenuation of host inflammation is not sufficient to restore CLV contraction during MRSA infection.
  • Fig. S4. Effect of MRSA infection on the blood vasculature.
  • Fig. S5. Characterization of LMCs, vascular muscle cells, and LECs.
  • Fig. S6. Identification of apoptotic cells in skin abscesses caused by MRSA infection.
  • Fig. S7. MRSA toxins contribute to reduced hSMC and LEC viability.
  • Legends for movies S1 to S4
  • Legends for tables S1 and S2
  • Reference (59)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Representative lymphatic contraction of afferent CLV to the PLN in uninfected mouse.
  • Movie S2 (.avi format). Representative lymphatic contraction of afferent CLV to the PLN in MRSA-infected (CA-MRSA USA300 JE2 strain) mouse 4 days after infection.
  • Movie S3 (.avi format). Representative lymphatic contraction of afferent CLV to the PLN in MRSA-infected (CA-MRSA USA300 strain) mouse 30 days after infection.
  • Movie S4 (.avi format). Representative lymphatic contraction of popliteal lymphatic vessel afferent CLV to the PLN in agr mutant–infected mouse 30 days after infection.
  • Table S1. List of proteins identified by mass spectrometry of supernatant from WT CA-MRSA USA300 JE2 strain (provided as an Excel file).
  • Table S2. Primary data (provided as an Excel file).

[Download Tables S1 and S2]