Supplementary Materials

Supplementary Material for:

Hippocampal extracellular matrix alterations contribute to cognitive impairment associated with a chronic depressive-like state in rats

Danai Riga, Ioannis Kramvis, Maija K. Koskinen, Pieter van Bokhoven, Johanneke E. van der Harst, Tim S. Heistek, A. Jaap Timmerman, Pim van Nierop, Roel C. van der Schors, Anton W. Pieneman, Anouk de Weger, Yvar van Mourik, Anton N. M. Schoffelmeer, Huib D. Mansvelder, Rhiannon M. Meredith, Witte J. G. Hoogendijk, August B. Smit, Sabine Spijker*

*Corresponding author. Email: s.spijker{at}

Published 13 December 2017, Sci. Transl. Med. 9, eaai8753 (2017)
DOI: 10.1126/scitranslmed.aai8753

This PDF file includes:

  • Materials and Methods
  • Fig. S1. The SDPS paradigm elicits physiological stress responses that subside after several weeks.
  • Fig. S2. The SDPS paradigm triggers imipramine-reversible reduction in LTP maintenance.
  • Fig. S3. Expression of synaptic proteins after SDPS.
  • Fig. S4. Representative immunoblots and corresponding loading control.
  • Fig. S5. Overall CSPG expression is not affected by SDPS.
  • Fig. S6. Cat-301 recognizes a CSPG-rich PNN population in hippocampus.
  • Fig. S7. Cat-301 recognizes aggrecan-rich PNNs, which increase after SDPS.
  • Fig. S8. CSPG-rich PNN characterization in dorsal hippocampus CA1 layers.
  • Fig. S9. SDPS does not affect PNN number in the perirhinal cortex.
  • Fig. S10. The effects of chondroitinase ABC on CSPGs and PNN recovery 2 weeks after administration.
  • Fig. S11. Chondroitinase ABC does not affect sIPSC amplitude.
  • Fig. S12. Extracellular matrix reorganization rescues SDPS-induced deficits in social recognition and mildly attenuates social withdrawal.
  • Table S1. SDPS-induced changes in dorsal hippocampus synaptic protein expression and rescue by the antidepressant imipramine.
  • Table S2. Overview of statistical tests used in the main figures.
  • References (7790)

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