Supplementary Materials

Supplementary Material for:

Human ESC–derived retinal epithelial cell sheets potentiate rescue of photoreceptor cell loss in rats with retinal degeneration

Karim Ben M'Barek, Walter Habeler, Alexandra Plancheron, Mohamed Jarraya, Florian Regent, Angélique Terray, Ying Yang, Laure Chatrousse, Sophie Domingues, Yolande Masson, José-Alain Sahel, Marc Peschanski, Olivier Goureau,* Christelle Monville*

*Corresponding author. Email: cmonville{at}istem.fr (C.M.); olivier.goureau{at}inserm.fr (O.G.)

Published 20 December 2017, Sci. Transl. Med. 9, eaai7471 (2017)
DOI: 10.1126/scitranslmed.aai7471

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Characterization of the RC-9 hESC line.
  • Fig. S2. Process for hESC-RPE cell generation.
  • Fig. S3. Expression of RPE markers by hESC-RPE cells.
  • Fig. S4. Purity of hESC-RPE cell preparation by expression of PAX-6 and MITF.
  • Fig. S5. Purity of hESC-RPE cell preparation by expression of cytokeratins.
  • Fig. S6. Evaluation of hESC-RPE cell preparation by FACS.
  • Fig. S7. Visualization of hESC-RPE cell polarity on the hAM scaffold by immunofluorescence.
  • Fig. S8. Analysis of hESC-RPE cells on the hAM scaffold by transmission and scanning electron microscopy.
  • Fig. S9. Phagocytosis by hESC-RPE cells.
  • Fig. S10. Absence of apoptosis in the hESC-RPE cell monolayer after extrusion from the injection device.
  • Fig. S11. Functional assessment in RCS rats after transplantation with hESC-RPE cell sheets compared to hAM or gelatin alone.
  • Fig. S12. OCT analysis of transplanted retinas.
  • Fig. S13. OCT analysis of retinas after transplantation with hESC-RPE cell sheets compared to hAM or gelatin alone.
  • Fig. S14. Analysis of inflammation markers in the retina of RCS rats after transplantation of gelatin alone.
  • Table S1. Quality control data for the hESC bank.
  • Table S2. Quality control data for the hESC-RPE bank.
  • Table S3. Targeted regions for virus detection.
  • Table S4. List of quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) probes.
  • Table S5. List of primary antibodies.
  • Legends for movies S1 to S3

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1. (.mp4 format). 3D reconstructed images of hESC-RPE cell sheets after phagocytosis of photoreceptor cell outer segments.
  • Movie S2. (.avi format). Method developed to load the hESC-RPE cell sheet into the injection device.
  • Movie S3. (.avi format). 3D reconstructed images of a thin section of normal retina transplanted with the hESC-RPE cell sheet.