Supplementary Materials

Supplementary Material for:

A reversible thermoresponsive sealant for temporary closure of ocular trauma

Niki Bayat, Yi Zhang, Paulo Falabella, Roby Menefee, John J. Whalen III,* Mark S. Humayun, Mark E. Thompson

*Corresponding author. Email: jjwhalen{at}

Published 6 December 2017, Sci. Transl. Med. 9, eaan3879 (2017)
DOI: 10.1126/scitranslmed.aan3879

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Schematic synthesis routes of the N85NT15 and N95BA5 copolymers.
  • Fig. S2. 1H NMR spectrum for N85NT15 and N95BA5 copolymers in CDCl3.
  • Fig. S3. Scattering intensity spectra of N95BA5 as a function of temperature.
  • Fig. S4. Strain amplitude for N95BA5 and N85NT15 at T = 6°C.
  • Fig. S5. Strain amplitude of N85NT15 at its LCST.
  • Fig. S6. Complex viscosity of N95BA5 as a function of temperature and concentration.
  • Fig. S7. Compressive stress-strain characterization and compressive modulus of the N95BA5 hydrogel.
  • Fig. S8. Tensile stress-strain characterization and tensile modulus of the N95BA5 hydrogel.
  • Fig. S9. Intensity distribution graph of DLS spectra for N95BA5 recorded at different temperatures.
  • Fig. S10. Hydrophobic/hydrophilic nature of the N95BA5 hydrogel above and below the LCST.
  • Fig. S11. Injector tool cooling reaction calibration curves for 2.5 and 12.5 g of ammonium nitrate to various volumes of added water.
  • Fig. S12. Visual evaluation of eye responses to surgical procedure and sealant placement.
  • Fig. S13. TRS in the hands of professionals: In vitro application at Walter Reed Medical Center.
  • Table S1. Injector tool design requirements.
  • Table S2. In vivo study tabulated trajectory.
  • Table S3. In vivo statistical analysis of average OD/OS for groups over time.
  • Table S4. Scleral tissue response at the hydrogel-sclera interface.
  • Table S5. Responses from freehand write-in section of user survey administered during clinical user workshop.
  • Table S6. Responses from multiple choice section of user survey administered during clinical user workshop.
  • Legend for movie S1

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). In vivo trauma simulation and hydrogel application procedure.