Supplementary Materials

Supplementary Material for:

BCAS1 expression defines a population of early myelinating oligodendrocytes in multiple sclerosis lesions

Maryam K. Fard,* Franziska van der Meer, Paula Sánchez, Ludovico Cantuti-Castelvetri, Sunit Mandad, Sarah Jäkel, Eugenio F. Fornasiero, Sebastian Schmitt, Marc Ehrlich, Laura Starost, Tanja Kuhlmann, Christina Sergiou, Verena Schultz, Claudia Wrzos, Wolfgang Brück, Henning Urlaub, Leda Dimou, Christine Stadelmann,* Mikael Simons*

*Corresponding author. Email: cstadelmann{at}med.uni-goettingen.de (C.S.); khojasteh{at}em.mpg.de (M.K.F.); msimons{at}gwdg.de (M.S.)

Published 6 December 2017, Sci. Transl. Med. 9, eaam7816 (2017)
DOI: 10.1126/scitranslmed.aam7816

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Validation of antiserum raised against BCAS1.
  • Fig. S2. BCAS1 specifically labels cells of oligodendrocyte lineage.
  • Fig. S3. Characterization of BCAS1+ pre-mOLGs and mOLGs.
  • Fig. S4. Characterization of ENPP6+ oligodendrocytes in mice.
  • Fig. S5. BCAS1 is down-regulated in fully matured oligodendrocytes prepared from mice.
  • Fig. S6. Comparison of BCAS1 with O1 and O4 immunolabeling.
  • Fig. S7. BCAS1 is down-regulated in fully matured oligodendrocytes prepared from human iPSCs.
  • Fig. S8. Dynamics of BCAS1+ cells generation in adult mouse cortex.
  • Fig. S9. Quantification of the number of BCAS1+ cells in the adult mouse brain.
  • Fig. S10. Developmentally born mOLGs continue myelination into late adulthood.
  • Fig. S11. BCAS1+ oligodendrocytes are distinct from PLP+ and ENPP6+ oligodendrocytes in the human brain.
  • Fig. S12. Characterization of BCAS1+ oligodendrocytes in the human brain.
  • Fig. S13. BCAS1+ oligodendroglia are rare in and around chronic demyelinated MS lesions and fully remyelinated shadow plaques.
  • Table S1. Clinical characteristics of patients to study the developmental expression of BCAS1.
  • Table S2. Clinical characteristics of MS patients.
  • References (46–48)

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Other Supplementary Material for this manuscript includes the following:

  • Table S3. (Microsoft Excel format). Primary data.