Supplementary Materials

Supplementary Material for:

Irisin protects mitochondria function during pulmonary ischemia/reperfusion injury

Ken Chen, Zaicheng Xu, Yukai Liu, Zhen Wang, Yu Li, Xuefei Xu, Caiyu Chen, Tianyang Xia, Qiao Liao, Yonggang Yao, Cindy Zeng, Duofen He, Yongjian Yang, Tao Tan, Jianxun Yi, Jingsong Zhou, Hua Zhu, Jianjie Ma,* Chunyu Zeng*

*Corresponding author. Email: chunyuzeng01{at}163.com (C.Z.); jianjie.ma{at}osumc.edu (J.M.)

Published 29 November 2017, Sci. Transl. Med. 9, eaao6298 (2017)
DOI: 10.1126/scitranslmed.aao6298

This PDF file includes:

  • Fig. S1. Specificity of irisin antibody and absence of endogenous irisin expression in lung epithelial cells.
  • Fig. S2. Mass spectrogram profile of irisin peptide.
  • Fig. S3. Irisin expression in lung tissues from sham-treated mice after RIPC.
  • Fig. S4. Irisin expression in mouse lung tissues at different time points after RIPC, with or without IR injury.
  • Fig. S5. OCR of lung tissue from IR-injured mice.
  • Fig. S6. Time-dependent accumulation of exogenous irisin in RLE-6TN and BEAS-2B cells.
  • Fig. S7. Localization of irisin in mitochondria of cultured lung epithelial cells.
  • Fig. S8. Measurement of cell viability and LDH concentration in culture medium derived from lung epithelial cells after AR.
  • Fig. S9. Colocalization of irisin and UCP2 in lung epithelial cells.
  • Fig. S10. Basal UCP2 expression, inflammation markers, and amount of ROS in WT and Ucp2−/− mice.
  • Table S1. The characteristics of control and NRDS newborn patients in Fig. 2B.
  • Table S2. The characteristics of control and NRDS newborn patients in Fig. 2C.
  • Legends for movies S1 to S3

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Other Supplementary Material for this manuscript includes the following:

  • Table S3. Original data and P values (Excel file).
  • Movie S1 (.wmv format). Uptake of exogenous irisin into BEAS-2B cells.
  • Movie S2 (.wmv format). Uptake of exogenous irisin into RLE-6TN cells.
  • Movie S3 (.wmv format). Uptake of exogenous irisin into A549 cells.

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[Download Movies S1 to S3]