Supplementary Materials

Supplementary Material for:

Prion seeding activity and infectivity in skin samples from patients with sporadic Creutzfeldt-Jakob disease

Christina D. Orrú, Jue Yuan, Brian S. Appleby, Baiya Li, Yu Li, Dane Winner, Zerui Wang, Yi-An Zhan, Mark Rodgers, Jason Rarick, Robert E. Wyza, Tripti Joshi, Gong-Xian Wang, Mark L. Cohen, Shulin Zhang, Bradley R. Groveman, Robert B. Petersen, James W. Ironside, Miguel E. Quiñones-Mateu, Jiri G. Safar, Qingzhong Kong,* Byron Caughey,* Wen-Quan Zou*

*Corresponding author. Email: wxz6{at}case.edu (W.-Q.Z.); bcaughey{at}nih.gov (B.C.); qingzhong.kong{at}case.edu (Q.K.)

Published 22 November 2017, Sci. Transl. Med. 9, eaam7785 (2017)
DOI: 10.1126/scitranslmed.aam7785

This PDF file includes:

  • Methods
  • Fig. S1. Western blotting and neurohistology of autopsy brain samples from sCJD and vCJD patients.
  • Fig. S2. Representative immunohistochemistry of skin samples from two vCJD patients and a non-CJD patient.
  • Fig. S3. Representative RT-QuIC end-point dilution analysis of brain and skin fractions from two CJD patients.
  • Fig. S4. Western blot analysis of bank vole rPrPres RT-QuIC products from reactions seeded with brain or skin samples.
  • Fig. S5. Western blot analysis of brain samples from TgNN6h or TgWV mice inoculated intracerebrally with sCJDMM2 or sCJDVV2 brain homogenate, respectively.
  • Table S1. Summary of non-CJD cases.

[Download PDF]