Supplementary Materials

Supplementary Material for:

TCR engagement negatively affects CD8 but not CD4 CAR T cell expansion and leukemic clearance

Yinmeng Yang, M. Eric Kohler, Christopher D. Chien, Christopher T. Sauter, Elad Jacoby, Chunhua Yan, Ying Hu, Kelsey Wanhainen, Haiying Qin, Terry J. Fry*

*Corresponding author. Email: fryt{at}mail.nih.gov

Published 22 November 2017, Sci. Transl. Med. 9, eaag1209 (2017)
DOI: 10.1126/scitranslmed.aag1209

This PDF file includes:

  • Fig. S1. CAR4 and CAR8 cells demonstrate comparable in vivo efficacy at low doses against murine ALL, E2aPbx.
  • Fig. S2. The presence of both TCR and CAR antigens diminishes efficacy of CAR8 but not CAR4 cells at 14 days.
  • Fig. S3. TCR stimulation induces down-regulation of CAR expression.
  • Fig. S4. Up-regulation of PD-1 and Lag3 expression is dependent on CAR stimulation.
  • Fig. S5. CAR8 cells are capable of endogenous B cell clearance despite ineffective leukemia clearance.
  • Fig. S6. In vitro CAR4 and CAR8 cell cytokine profiles when stimulated through TCR, CAR, or both receptors.
  • Fig. S7. Gene list for pathways/gene sets from CAR4 and CAR8 cells stimulated through CAR, TCR, or both receptors.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Primary data.

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