Supplementary Materials

Supplementary Material for:

Apoptosis in mesenchymal stromal cells induces in vivo recipient-mediated immunomodulation

Antonio Galleu, Yanira Riffo-Vasquez, Cristina Trento, Cara Lomas, Luigi Dolcetti, Tik Shing Cheung, Malte von Bonin, Laura Barbieri, Krishma Halai, Sophie Ward, Ling Weng, Ronjon Chakraverty, Giovanna Lombardi, Fiona M. Watt, Kim Orchard, David I. Marks, Jane Apperley, Martin Bornhauser, Henning Walczak, Clare Bennett, Francesco Dazzi*

*Corresponding author. Email: francesco.dazzi{at}kcl.ac.uk

Published 15 November 2017, Sci. Transl. Med. 9, eaam7828 (2017)
DOI: 10.1126/scitranslmed.aam7828

This PDF file includes:

  • Materials and Methods
  • Fig. S1. MSCs can be traced in the lungs of mice after infusion.
  • Fig. S2. Human MSC immunosuppression is not licensed by murine cytokines.
  • Fig. S3. MSC apoptosis is induced by cytotoxic cells.
  • Fig. S4. Cytotoxicity against MSCs varies among PBMC donors but is independent on the percentage of CD8+ or CD56+ in GvHD patients.
  • Fig. S5. MSC killing is mediated by caspase 3 and effected by GrB and perforin.
  • Fig. S6. Infused MSCs can be imaged in the lungs of mice with TH2-type lung inflammation.
  • Table S1. Clinical features of GvHD patients.
  • Table S2. Primary data.
  • Legends for videos S1 to S6
  • References (5557)

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Other Supplementary Material for this manuscript includes the following:

  • Video S1 (.avi format). Living-cell imaging of fluorescence resonance energy transfer (FRET)–MSCs plated alone.
  • Video S2 (.avi format). Living-cell imaging of FRET-MSCs plated with PHA-aPBMCs.
  • Video S3 (.avi format). Living-cell imaging of FRET-MSCs plated with resting PBMCs.
  • Video S4 (.avi format). Living-cell imaging of FRET-MSCs plated with PHA-aPBMCs in the presence of the pan-caspase inhibitor Z-VAD-FMK.
  • Video S5 (.avi format). Living-cell imaging of FRET-MSCs plated with PHA-aPBMCs in the presence of the GrB inhibitor Z-AAD-CMK.
  • Video S6 (.avi format). Living-cell imaging of FRET-MSCs plated with PHA-aPBMCs in the presence of the perforin inhibitor EGTA.

[Download Videos S1 to S6]