Supplementary Materials

Supplementary Material for:

PD-L1 genetic overexpression or pharmacological restoration in hematopoietic stem and progenitor cells reverses autoimmune diabetes

Moufida Ben Nasr, Sara Tezza, Francesca D'Addio, Chiara Mameli, Vera Usuelli, Anna Maestroni, Domenico Corradi, Silvana Belletti, Luca Albarello, Gabriella Becchi, Gian Paolo Fadini, Christian Schuetz, James Markmann, Clive Wasserfall, Leonard Zon, Gian Vincenzo Zuccotti, Paolo Fiorina*

*Corresponding author. Email: paolo.fiorina{at}childrens.harvard.edu

Published 15 November 2017, Sci. Transl. Med. 9, eaam7543 (2017)
DOI: 10.1126/scitranslmed.aam7543

This PDF file includes:

  • Materials and Methods
  • Fig. S1. PD-L1 expression in non-HSPCs.
  • Fig. S2. Generation of PD-L1.Tg KL cells and their tracking.
  • Fig. S3. Immunophenotype of pKL cells.
  • Fig. S4. Anti–PD-1 studies.
  • Fig. S5. CD34+ cells characterization in T1D patients and in healthy controls.
  • Fig. S6. Immunophenotype of pCD34+.
  • Fig. S7. Transcriptome of pCD34+.
  • Fig. S8. CD34+ cell mobilization with plerixafor and working hypothesis.
  • Legends for tables S1 and S2
  • Table S3. Genome-wide expression analysis of Tg KL cells.
  • Table S4. Chemokine receptors expression in different groups of KL cells.
  • Legends for tables S5 and S6
  • Table S7. Characteristics of patients enrolled in the study.
  • Table S8. Characteristics of patients enrolled in the plerixafor mobilization study.
  • Legends for tables S9 and S10

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Table S1. Transcriptomic profiling of murine KLS cells (provided as an Excel file).
  • Table S2. Genome-wide expression analysis of murine KLS cells (provided as an Excel file).
  • Table S5. Genome-wide expression analysis of pKL cells: up-regulated genes (provided as an Excel file).
  • Table S6. Genome-wide expression analysis of pKL cells: down-regulated genes (provided as an Excel file).
  • Table S9. Transcriptome of pCD34+ cells (provided as an Excel file).
  • Table S10. Primary data (provided as an Excel file).

[Download Supplementary Tables]