Supplementary Materials

Supplementary Material for:

A role for bacterial urease in gut dysbiosis and Crohn's disease

Josephine Ni, Ting-Chin David Shen, Eric Z. Chen, Kyle Bittinger, Aubrey Bailey, Manuela Roggiani, Alexandra Sirota-Madi, Elliot S. Friedman, Lillian Chau, Andrew Lin, Ilana Nissim, Justin Scott, Abigail Lauder, Christian Hoffmann, Gloriany Rivas, Lindsey Albenberg, Robert N. Baldassano, Jonathan Braun, Ramnik J. Xavier, Clary B. Clish, Marc Yudkoff, Hongzhe Li, Mark Goulian, Frederic D. Bushman, James D. Lewis, Gary D. Wu*

*Corresponding author. Email: gdwu{at}pennmedicine.upenn.edu

Published 15 November 2017, Sci. Transl. Med. 9, eaah6888 (2017)
DOI: 10.1126/scitranslmed.aah6888

This PDF file includes:

  • Extended experimental procedures
  • Fig. S1. Fecal amino acids in healthy control subjects versus patients with pediatric Crohn’s disease.
  • Fig. S2. Composition of the gut microbiota at baseline and 1 month after ABX/PEG bowel cleansing in three independent experiments.
  • Fig. S3. Test for difference in microbiota community composition based on unweighted UniFrac distance.
  • Fig. S4. Heat map and Kruskal-Wallis analysis of the murine gut microbiota before and after inoculation with Ure and Ure+ E. coli MP1.
  • Fig. S5. Changes in microbiota composition and fecal amino acids in mice inoculated with Ure+ versus Ure E. coli MP1 in a T cell adoptive transfer model of colitis.

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