Supplementary Materials

Supplementary Material for:

Matrix-binding checkpoint immunotherapies enhance antitumor efficacy and reduce adverse events

Jun Ishihara, Kazuto Fukunaga, Ako Ishihara, Hans M. Larsson, Lambert Potin, Peyman Hosseinchi, Gabriele Galliverti, Melody A. Swartz, Jeffrey A. Hubbell*

*Corresponding author. Email: jhubbell{at}uchicago.edu

Published 8 November 2017, Sci. Transl. Med. 9, eaan0401 (2017)
DOI: 10.1126/scitranslmed.aan0401

This PDF file includes:

  • Fig. S1. MALDI-TOF MS analysis revealed that molecular weights are increased by PlGF-2123–144 conjugation to αCTLA4 (4F10) and αPD-L1.
  • Fig. S2. PlGF-2123–144 conjugation to αCTLA4 (9H10) and αPD-L1 does not yield aggregates.
  • Fig. S3. Unmodified αCTLA4 (4F10) and αPD-L1 do not bind to ECM proteins.
  • Fig. S4. Affinities of PlGF-2123–144–αCTLA4 and PlGF-2123–144–αPD-L1 for fibronectin and collagen I were measured.
  • Fig. S5. Affinities of PlGF-2123–144–αCTLA4 or unmodified αCTLA4 and αPD-L1 for their antigens were measured.
  • Fig. S6. Heparin inhibits fibronectin binding of PlGF-2123–144–Abs.
  • Fig. S7. Plasmin cleaves PlGF-2123–144.
  • Fig. S8. PlGF-2123–144–Abs are retained in collagen in vitro.
  • Fig. S9. PlGF-2123–144–Abs are retained in the melanoma tissue.
  • Fig. S10. PlGF-2123–144 conjugation does not affect binding of αPD-L1 and FcRn receptor.
  • Fig. S11. Liver histologies after unmodified and PlGF-2123–144–checkpoint blockade Ab treatment are shown.
  • Fig. S12. PlGF-2123–144–Ab treatment extends survival of B16F10 tumor-bearing mice.
  • Fig. S13. Single-agent treatment with PlGF-2123–144–αCTLA4 or PlGF-2123–144–αPD-L1 does not affect tumor growth.
  • Fig. S14. PlGF-2123–144 peptide does not affect B16F10 cells’ proliferation.
  • Fig. S15. PlGF-2123–144–Ab treatment activates T cells in the spleen.
  • Fig. S16. PlGF-2123–144–Ab treatment activates tumor antigen–specific T cells in tdLN, tested in the B16F10-OVA model.
  • Fig. S17. Individual tumor growth curves for Fig. 5 are shown.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 Original data (provided as an Excel file).