Supplementary Materials

Supplementary Material for:

A distinct hematopoietic stem cell population for rapid multilineage engraftment in nonhuman primates

Stefan Radtke, Jennifer E. Adair, Morgan A. Giese, Yan-Yi Chan, Zachary K. Norgaard, Mark Enstrom, Kevin G. Haworth, Lauren E. Schefter, Hans-Peter Kiem*

*Corresponding author. Email: hkiem{at}fredhutch.org

Published 1 November 2017, Sci. Transl. Med. 9, eaan1145 (2017)
DOI: 10.1126/scitranslmed.aan1145

This PDF file includes:

  • Methods
  • Fig. S1. Engraftment kinetics in transplanted nonhuman primates.
  • Fig. S2. HSPC composition in nonhuman primate stem cell populations from different sources.
  • Fig. S3. Ex vivo culture of nonhuman primate HSPCs.
  • Fig. S4. T cell potential of nonhuman primate HSPCs.
  • Fig. S5. Lympho-myeloid potential of nonhuman primate HSPCs.
  • Fig. S6. Colony-forming cell potential of nonhuman primate HSPCs.
  • Fig. S7. Megakaryocyte potential of nonhuman primate HSPCs.
  • Fig. S8. Cobblestone area–forming cell potential of nonhuman primate HSPCs.
  • Fig. S9. Secondary colony-forming cell potential of nonhuman primate HSPCs.
  • Fig. S10. Quality control for competitive repopulation experiments.
  • Fig. S11. Neutrophil recovery in transplanted nonhuman primates.
  • Fig. S12. Platelet recovery in transplanted nonhuman primates.
  • Fig. S13. Bone marrow engraftment of gene-modified HSPCs.
  • Fig. S14. Long-term engraftment versus failure.
  • Fig. S15. Correlation of recovery with infused HSPCs and colony-forming cells.
  • Fig. S16. Gating of HSC-enriched cell fractions in human umbilical cord blood.
  • Table S1. Identification of clones displaying HSC biology in vivo.
  • Table S2. Cross-species reactive antibodies in nonhuman primates.
  • Table S3. MS-5 assay colonies.
  • Table S4. Characteristics of nonhuman primate transplants.
  • Table S5. Characteristics of nonhuman primate transplants II.
  • Table S6. Differentially expressed genes in human and nonhuman primate HSCs.
  • References (4054)

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