Supplementary Materials

Supplementary Material for:

Overcoming mutational complexity in acute myeloid leukemia by inhibition of critical pathways

Yoriko Saito, Yoshiki Mochizuki, Ikuko Ogahara, Takashi Watanabe, Leah Hogdal, Shinsuke Takagi, Kaori Sato, Akiko Kaneko, Hiroshi Kajita, Naoyuki Uchida, Takehiro Fukami, Leonard D. Shultz, Shuichi Taniguchi, Osamu Ohara, Anthony G. Letai, Fumihiko Ishikawa*

*Corresponding author. Email: fumihiko.ishikawa{at}riken.jp

Published 25 October 2017, Sci. Transl. Med. 9, eaao1214 (2017)
DOI: 10.1126/scitranslmed.aao1214

This PDF file includes:

  • Fig. S1. Identification of hematopoietic subpopulations in patient samples by surface phenotype and in vivo function in three additional patients.
  • Fig. S2. Mutational profiles of AML cells with and without in vivo RK-20449 treatment.
  • Fig. S3. Mutational profiles of AML patient samples obtained serially during clinical course.
  • Fig. S4. Altered transcription profiles of human AML cells with in vivo RK-20449 treatment.
  • Fig. S5. Overcoming genetically complex AML by targeting leukemia-initiating mutation and antiapoptotic BCL-2 pathway.
  • Legends for tables S1 to S8
  • Reference (45)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1. Patient characteristics (provided as an Excel file).
  • Table S2. Information of identified variants (provided as an Excel file).
  • Table S3. Human AML chimerism in the PB, BM, and spleen of AML-engrafted recipients treated with RK-20449 alone (provided as an Excel file).
  • Table S4. Human AML chimerism in the PB, BM, and spleen of AML-engrafted recipients treated with ABT-199 alone, RK-20449 alone, or combination (provided as an Excel file).
  • Table S5. Statistics comparing pre- and posttreatment PB human AML chimerism in recipients treated with ABT-199 alone, RK-20449 alone, or combination (provided as an Excel file).
  • Table S6. Statistics comparing BM and spleen human AML chimerism in recipients treated with ABT-199 alone, RK-20449 alone, or combination (provided as an Excel file).
  • Table S7. Effect of in vivo exposure to combined RK-20449 and ABT-199 on human multilineage hematopoiesis (provided as an Excel file).
  • Table S8. PCR primers for targeted sequencing of MALBAC products (provided as an Excel file).

[Download Tables S1 to S8]