Supplementary Materials

Supplementary Material for:

An analysis of IL-36 signature genes and individuals with IL1RL2 knockout mutations validates IL-36 as a psoriasis therapeutic target

Satveer K. Mahil, Marika Catapano, Paola Di Meglio, Nick Dand, Helena Ahlfors, Ian M. Carr, Catherine H. Smith, Richard C. Trembath, Mark Peakman, John Wright, Francesca D. Ciccarelli, Jonathan N. Barker, Francesca Capon*

*Corresponding author. Email: francesca.capon{at}kcl.ac.uk

Published 11 October 2017, Sci. Transl. Med. 9, eaan2514 (2017)
DOI: 10.1126/scitranslmed.aan2514

This PDF file includes:

  • Materials and Methods
  • Fig. S1. IL-4 target genes are an appropriate negative control for IL-36 enrichment analyses, because there is no overlap between IL-4 and IL-36 signature genes.
  • Fig. S2. The pathways that underlie the pathogenesis of GPP are enriched within the IL-36 transcriptome.
  • Fig. S3. Mechanical trauma up-regulates IL-36 production by cultured keratinocytes.
  • Fig. S4. Proposed pathogenic model illustrating the role of IL-36 as an amplifier of TH17 signaling in psoriasis.
  • Fig. S5. In vivo IL-36 signaling blockade reduces the infiltration of IL-17–producing αβ T cells in imiquimod-induced psoriasiform dermatitis.
  • Fig. S6. The genes encoding the IL-36R subunits are expressed in PBMCs.
  • Fig. S7. Dose- and time-dependent effects of IL-36 cytokines in primary keratinocytes.
  • Fig. S8. Complete Western blots for Fig. 5E.
  • Table S5. Rare homozygous IL1RL2 variants observed in the Born in Bradford cohort.
  • Table S6. Full blood counts of individuals with homozygous IL1RL2 mutations and controls.
  • Table S7. Immune serology results.
  • Table S8. Skin donor demographics.
  • Table S9. Oligonucleotide primers used in the study.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1. RNA-seq coverage statistics (provided in Excel).
  • Table S2. Keratinocyte genes that are up-regulated or down-regulated by IL-36 treatment (provided in Excel).
  • Table S3. Overlap between the IL36_182 set and keratinocyte genes up-regulated by IL-17 (provided in Excel).
  • Table S4. Results of pathway enrichment analysis (provided in Excel).
  • Table S10. Raw data for in vivo and ex vivo experiments (provided in Excel).

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