Supplementary Materials

Supplementary Material for:

Rapid antigen tests for dengue virus serotypes and Zika virus in patient serum

Irene Bosch, Helena de Puig, Megan Hiley, Marc Carré-Camps, Federico Perdomo-Celis, Carlos F. Narváez, Doris M. Salgado, Dewahar Senthoor, Madeline O'Grady, Elizabeth Phillips, Ann Durbin, Diana Fandos, Hikaru Miyazaki, Chun-Wan Yen, Margarita Gélvez-Ramírez, Rajas V. Warke, Lucas S. Ribeiro, Mauro M. Teixeira, Roque P. Almeida, José E. Muñóz-Medina, Juan E. Ludert, Mauricio L. Nogueira, Tatiana E. Colombo, Ana C. B. Terzian, Patricia T. Bozza, Andrea S. Calheiros, Yasmine R. Vieira, Giselle Barbosa-Lima, Alexandre Vizzoni, José Cerbino-Neto, Fernando A. Bozza, Thiago M. L. Souza, Monique R. O. Trugilho, Ana M. B. de Filippis, Patricia C. de Sequeira, Ernesto T. A. Marques, Tereza Magalhaes, Francisco J. Díaz, Berta N. Restrepo, Katerine Marín, Salim Mattar, Daniel Olson, Edwin J. Asturias, Mark Lucera, Mohit Singla, Guruprasad R. Medigeshi, Norma de Bosch, Justina Tam, Jose Gómez-Márquez, Charles Clavet, Luis Villar, Kimberly Hamad-Schifferli,* Lee Gehrke*

*Corresponding author. Email: kim.hamad{at}umb.edu (K.H.-S.); lgehrke{at}mit.edu (L.G.)

Published 27 September 2017, Sci. Transl. Med. 9, eaan1589 (2017)
DOI: 10.1126/scitranslmed.aan1589

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Stepwise strategy for identifying mAbs that differentiate the closely related NS1 proteins of DENV1–4 and ZIKV.
  • Fig. S2. Repertoire of antigen-specific antibodies among DENV and ZIKV mAbs.
  • Fig. S3. Limit of detection comparison.
  • Fig. S4. The Standard Diagnostics DENV NS1 test cross-reacts with ZIKV NS1 protein.
  • Fig. S5. DENV NS1 and ZIKV NS1 detection expressed as days after onset of fever symptoms.
  • Fig. S6. Laboratory-made gold nanoparticles for detecting DENV NS1 in rapid test format.
  • Fig. S7. NS1 detection by rapid tests in secondary infections.
  • Table S1. Stepwise description of the approaches used to define antibodies that detect and distinguish DENV1–4 NS1 and the ZIKV NS1 proteins.
  • Table S2. Amino acid homology and identity among DENV NS1 and ZIKV NS1 proteins.
  • Table S3. Matrix of mAb pair trials.
  • Table S4. List of mAbs used in the rapid tests, relative binding values, and summary of final use in the DENV and ZIKV immunochromatography tests.
  • References (47, 48)

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Other Supplementary Material for this manuscript includes the following:

  • Table S5 (Microsoft Excel format). Individual subject-level data.

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