Supplementary Materials

Supplementary Material for:

RNAi-based treatment of chronically infected patients and chimpanzees reveals that integrated hepatitis B virus DNA is a source of HBsAg

Christine I. Wooddell,* Man-Fung Yuen, Henry Lik-Yuen Chan, Robert G. Gish, Stephen A. Locarnini, Deborah Chavez, Carlo Ferrari, Bruce D. Given, James Hamilton, Steven B. Kanner, Ching-Lung Lai, Johnson Y. N. Lau, Thomas Schluep, Zhao Xu, Robert E. Lanford, David L. Lewis

*Corresponding author. Email: cwooddell{at}arrowheadpharma.com

Published 27 September 2017, Sci. Transl. Med. 9, eaan0241 (2017)
DOI: 10.1126/scitranslmed.aan0241

This PDF file includes:

  • Materials and Methods
  • Fig. S1. HBV sequence elements near DR1 and DR2.
  • Fig. S2. Serum HBsAg, HBeAg, and HBcrAg reduction in human patients treated with two doses of ARC-520 2 weeks apart.
  • Fig. S3. Reduction of HBV serum DNA in CHB patients treated with a single dose of ARC-520 on a background of NUCs.
  • Fig. S4. Quantitation of liver HBV DNA in chimpanzees.
  • Fig. S5. Mapping of HBV DNA integration sites.
  • Fig. S6. Massive paired-end sequencing of chimpanzee liver mRNA and alignment to HBV reference genome.
  • Fig. S7. Immunohistochemical staining of HBsAg in livers of HBeAg-positive and HBeAg-negative chimpanzees.
  • Table S1. Individual CHB patient demographics at screening and maximum HBsAg reduction from baseline in clinical trial Heparc-2001.
  • Table S2. Mean CHB patient demographics at screening and maximum HBsAg reduction from baseline in clinical trial Heparc-2001.
  • Table S3. Treatment-emergent adverse events in cohorts 1 to 7 of the Heparc-2001 study.
  • Table S4. Characteristics of chimpanzees chronically infected with HBV and treatment regimens.
  • Table S5. mRNA-seq data obtained from liver biopsies of chimpanzees chronically infected with HBV.
  • Table S6. PASs in HBV-chimpanzee fusion transcripts of HBeAg-negative chimpanzee 88A010.
  • Table S7. HBV genome characteristics determined from liver DNA sequencing of study chimpanzees.

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Other Supplementary Material for this manuscript includes the following:

  • Table S8 (Microsoft Excel format). Primary data.

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