Supplementary Materials

Supplementary Material for:

Neutrophil macroaggregates promote widespread pulmonary thrombosis after gut ischemia

Yuping Yuan, Imala Alwis, Mike C. L. Wu, Zane Kaplan, Katrina Ashworth, David Bark Jr., Alan Pham, James Mcfadyen, Simone M. Schoenwaelder, Emma C. Josefsson, Benjamin T. Kile, Shaun P. Jackson*

*Corresponding author. Email: shaun.jackson{at}sydney.edu.au

Published 27 September 2017, Sci. Transl. Med. 9, eaam5861 (2017)
DOI: 10.1126/scitranslmed.aam5861

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Formation of leukocyte aggregates and fibrin in the pulmonary vasculature of mice after gut I/R injury.
  • Fig. S2. Neutrophil aggregate and fibrin formation in the lung vasculature of I/R-injured mice and ARDS patients.
  • Fig. S3. Neutrophil aggregate formation in the splanchnic circulation of the mouse after gut I/R injury.
  • Fig. S4. P-selectin–expressing platelets on gut vasculature and within neutrophil aggregates after gut I/R injury.
  • Fig. S5. Neutrophil aggregation requires potent platelet activation.
  • Fig. S6. PS+ platelets selectively support neutrophil aggregation in vivo.
  • Fig. S7. Occlusive neutrophil aggregate formation is associated with fibrin formation.
  • Fig. S8. Rolling neutrophils pull membrane fragments from PS+ platelets.
  • Fig. S9. Characterization of platelets deficient in CypD or Bak/Bax.
  • Fig. S10. Conventional antiplatelet therapies do not prevent neutrophil aggregate formation after gut I/R injury.
  • Fig. S11. Schematic illustration of the proposed mechanism linking gut I/R injury to pulmonary thrombosis.
  • Table S1. ARDS: Patient details.
  • Table S2. APE: Patient details.
  • Legends for movies S1 to S5
  • References (6773)

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Other Supplementary Material for this manuscript includes the following:

  • Table S3. Primary data (Excel file).
  • Movie S1 (.mov format). Neutrophil aggregate formation on PS+ thrombi at sites of vascular injury.
  • Movie S2 (.mov format). PS+ platelet-neutrophil aggregate formation in the mesenteric veins after gut I/R injury.
  • Movie S3 (.mov format). Neutrophil ripping and dragging PS+ platelet membranes by rolling neutrophils in vitro (low magnification).
  • Movie S4 (.mov format). Neutrophil ripping PS+ platelets in vivo and in vitro.
  • Movie S5 (.mov format). Neutrophil extract PS+ platelets in the intestinal vasculature after gut I/R injury.