Supplementary Materials

Supplementary Material for:

Human pluripotent stem cell–derived erythropoietin-producing cells ameliorate renal anemia in mice

Hirofumi Hitomi, Tomoko Kasahara, Naoko Katagiri, Azusa Hoshina, Shin-Ichi Mae, Maki Kotaka, Takafumi Toyohara, Asadur Rahman, Daisuke Nakano, Akira Niwa, Megumu K. Saito, Tatsutoshi Nakahata, Akira Nishiyama, Kenji Osafune*

*Corresponding author. Email: osafu{at}

Published 27 September 2017, Sci. Transl. Med. 9, eaaj2300 (2017)
DOI: 10.1126/scitranslmed.aaj2300

This PDF file includes:

  • Fig. S1. Differentiation method for generating EPO-producing cells from hiPSCs/ESCs.
  • Fig. S2. Expression of hepatic lineage and endoderm markers in hiPSC-EPO cells.
  • Fig. S3. Variable expression and secretion of EPO and expression of hepatoblast markers among three hiPSC/ESC lines.
  • Fig. S4. Differentiation method for generating EPO-producing cells from miPSCs/ESCs.
  • Fig. S5. Effects of 46 different factors on EPO mRNA expression in the hiPSC-EPO cells.
  • Fig. S6. The HIF-PHD pathway regulates EPO production induced by hypoxia or IGF-1 treatment.
  • Fig. S7. Effects of hiPSC-EPO protein on body weight and renal anemia in adenine-treated mice.
  • Table S1. Effects of hiPSC-EPO protein on in vitro erythropoiesis (related to Fig. 6).
  • Table S2. The sequences of sense and antisense primers used for RT-PCR in this study.
  • Table S3. A list of lectins and their specificity for microarray analysis.

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