Supplementary Materials

Supplementary Material for:

Responsive monitoring of mitochondrial redox states in heart muscle predicts impending cardiac arrest

Dorothy A. Perry, Joshua W. Salvin, Padraic Romfh, Peili Chen, Kalyani Krishnamurthy, Lindsay M. Thomson, Brian D. Polizzotti, Francis X. McGowan, Daryoosh Vakhshoori,* John N. Kheir*

*Corresponding author. Email: dvakhshoori{at}pendar.tech (D.V.); john.kheir{at}childrens.harvard.edu (J.N.K.)

Published 20 September 2017, Sci. Transl. Med. 9, eaan0117 (2017)
DOI: 10.1126/scitranslmed.aan0117

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Schematic of the RRS system.
  • Fig. S2. Sensitivity and specificity plots of 3RMR to detect severe tissue hypoxia.
  • Fig. S3. Survival curves over time for rodents during the hypoxia experiment when risk-stratified at 10 min using tissue PO2, tissue oxyhemoglobin saturation, or tissue oxymyoglobin saturation.
  • Fig. S4. Intracellular ATP and NADH concentrations after hypoxia experiment.
  • Fig. S5. Oxyhemoglobin and oxymyoglobin saturations in rodents separated by the 3RMR value.
  • Fig. S6. Raw RR spectra after baseline correction, the best-fit line of the explained spectrum, and the residual.
  • Fig. S7. Correlation between oxyhemoglobin saturations based on RR spectral analysis and traditional co-oximetry.
  • Fig. S8. Screenshot of the user interface.
  • Fig. S9. Quantile-quantile plots of the residual from the in vivo experiments.
  • Fig. S10. Measured spectra and residua from the in vivo experiments.
  • Fig. S11. Simultaneous changes in a single 3RMR and oxymyoglobin concentration reading are independent of changes in the total mitochondria–to–total myoglobin ratio.
  • Fig. S12. Distribution of bootstrap estimates from single–point-in-time measurements during baseline and severe hypoxia.
  • Table S1. Spectroscopically active components of myocardium.
  • Table S2. Baseline characteristics of rodents included in the hypoxia experiment.
  • Table S3. Depth of myocardial tissue sampling within a clinically relevant range of hemoglobin concentration and oxyhemoglobin saturation.
  • Table S4. Scale factors for hemoglobin, myoglobin, and mitochondria.
  • Table S5. Confidence limits of SHbO2, SMbO2, and 3RMR from a single instantaneous measurement during baseline and hypoxic conditions in a single animal.
  • References (5761)

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Other Supplementary Material for this manuscript includes the following:

  • Table S6 (Microsoft Excel format). Individual subject-level data.

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