Supplementary Materials

Supplementary Material for:

Endothelial APLNR regulates tissue fatty acid uptake and is essential for apelin's glucose-lowering effects

Cheol Hwangbo, Jingxia Wu, Irinna Papangeli, Takaomi Adachi, Bikram Sharma, Saejeong Park, Lina Zhao, Hyekyung Ju, Gwang-woong Go, Guoliang Cui, Mohammed Inayathullah, Judith K. Job, Jayakumar Rajadas, Stephanie L. Kwei, Ming O. Li, Alan R. Morrison, Thomas Quertermous, Arya Mani, Kristy Red-Horse, Hyung J. Chun*

*Corresponding author. Email: hyung.chun{at}yale.edu

Published 13 September 2017, Sci. Transl. Med. 9, eaad4000 (2017)
DOI: 10.1126/scitranslmed.aad4000

This PDF file includes:

  • Fig. S1. APLNR expression profiling.
  • Fig. S2. Fat and muscle content of AplnrECKO mice.
  • Fig. S3. Plasma insulin concentrations in Apln−/−, Aplnr−/−, and AplnrECKO mice.
  • Fig. S4. Adiponectin concentration in AplnrECKO mice.
  • Fig. S5. Hindlimb blood flow in AplnrECKO mice.
  • Fig. S6. Effect of apelin on transendothelial glucose transfer.
  • Fig. S7. Metabolic effects of high-fat diet.
  • Fig. S8. Effect of high glucose on APLN.
  • Fig. S9. Gene expression profiling of ECs subjected to APLN and APLNR knockdown or FOXO1 knockdown.
  • Fig. S10. FABP4 expression in response to APLN or FOXO1 knockdown.
  • Fig. S11. FABP4 expression in response to APLN and PPARG knockdown.
  • Fig. S12. FABP4 immunostaining in Apln−/− and Apln−/−:Foxo1ECKO mice.
  • Fig. S13. Effect of continuous apelin infusion on glucose tolerance testing.
  • Fig. S14. FABP4 expression in mice receiving apelin infusion.
  • Fig. S15. FA uptake in HUVECs subjected to modulation of apelin/APLNR and FABP4 activity.
  • Fig. S16. FA uptake profile of Foxo1ECKO mice.
  • Fig. S17. Serum FA concentrations in Apln−/− and Foxo1ECKO mice.
  • Fig. S18. Circulating serum FA concentrations in AplnrECKO mice.
  • Fig. S19. FA and triglyceride content of skeletal muscle of AplnrECKO mice.
  • Fig. S20. Endothelial permeability in the lung and skeletal muscle of AplnrECKO mice.
  • Fig. S21. Endothelial permeability in response to APLN and APLNR knockdown.
  • Fig. S22. Effect of pharmacologic FABP4 inhibition on glucose tolerance test in wild-type mice on normal chow.
  • Fig. S23. Efficacy of Aplnr deletion.
  • Fig. S24. Efficacy of Foxo1 gene deletion.
  • Fig. S25. Efficacy of EC purification from mouse tissues.
  • Fig. S26. Efficacy of APLN and PPARG knockdown.
  • Fig. S27. Efficacy of APLNR knockdown.
  • Table S1. Comparison of APLN/APLNR or FOXO1 knockdown to previous FOXO1/FOXO3A study.

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