Supplementary Materials

Supplementary Material for:

Synthetic oligosaccharides can replace animal-sourced low–molecular weight heparins

Yongmei Xu, Kasemsiri Chandarajoti, Xing Zhang, Vijayakanth Pagadala, Wenfang Dou, Debra Moorman Hoppensteadt, Erica M. Sparkenbaugh, Brian Cooley, Sharon Daily, Nigel S. Key, Diana Severynse-Stevens, Jawed Fareed, Robert J. Linhardt,* Rafal Pawlinski,* Jian Liu*

*Corresponding author. Email: jian_liu{at}unc.edu (J.L.); rafal_pawlinski{at}med.unc.edu (R.P.); linhar{at}rpi.edu (R.J.L.)

Published 6 September 2017, Sci. Transl. Med. 9, eaan5954 (2017)
DOI: 10.1126/scitranslmed.aan5954

This PDF file includes:

  • Materials and Methods
    Fig. S1. Chemoenzymatic synthetic scheme for 12-mer-1 and 12-mer-2.
    Fig. S2. Anion exchange HPLC chromatograms of 12-mer-1 and 12-mer-2.
    Fig. S3. High-resolution MS analysis of 12-mer-1 and 12-mer-2 by HILIC-FT
    MS.
    Fig. S4. 1H and 13C NMR spectra of 12-mer-1.
    Fig. S5. 1H-1H COSY and 1H-13C HSQC NMR spectra of 12-mer-1.
    Fig. S6. 1H-1H NOESY and 1H-1H TOCSY NMR spectra of 12-mer-1.
    Fig. S7. 1H-13C HMBC spectrum of 12-mer-1.
    Fig. S8. 1H NMR/13C NMR chemical shift assignments (in ppm) of 12-mer-1.
    Fig. S9. 1H and 13C NMR spectra of 12-mer-2.
    Fig. S10. 1H-1H COSY and 1H-13C HSQC spectra of 12-mer-2.
    Fig. S11. 1H-1H NOESY and 1H-1H TOCSY spectra of 12-mer-2.
    Fig. S12. 1H-13C HMBC spectrum of 12-mer-2.
    Fig. S13. 1H NMR/13C NMR chemical shift assignments (in ppm) of 12-mer-2.
    Fig. S14. Determining the structure of 12-mer-2 by heparin lyase II digestion.
    Fig. S15. Determination of the 3-O-sulfo group in 12-mer-2 by partial heparin
    lyase II digestion.
    Fig. S16. Confirmation of the location of the 3-O-sulfo group in 12-mer-2 by
    partial heparin lyase I digestion.
    Fig. S17. Comparison of 2D 1H-1H COSY with 1H NMR.
    Fig. S18. Comparison of 2D 1H-13C HSQC of 12-mer-1 and 12-mer-2.
    Fig. S19. Comparison of 2D 1H-1H TOCSY and NOESY spectra of 12-mer-2.
    Fig. S20. The effect of 12-mer-1 on the activity of FXa in mice.
    Fig. S21. In vitro assay method and standard curve of 12-mer-1 for pharmacodynamic studies in nonhuman primates.
    Fig. S22. Elimination profiles of 12-mer-1 in primate through intravenous or subcutaneous administration at 500 μg/kg.
    Table S1. Additional toxicological data and organ and body weight ratios.
    References (5257)

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