Supplementary Materials

Supplementary Material for:

New class of precision antimicrobials redefines role of Clostridium difficile S-layer in virulence and viability

Joseph A. Kirk, Dana Gebhart, Anthony M. Buckley, Stephen Lok, Dean Scholl, Gillian R. Douce, Gregory R. Govoni,* Robert P. Fagan*

*Corresponding author. Email: ggovons{at}gmail.com (G.R.G.); r.fagan{at}sheffield.ac.uk (R.P.F.)

Published 6 September 2017, Sci. Transl. Med. 9, eaah6813 (2017)
DOI: 10.1126/scitranslmed.aah6813

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Characterization of growth.
  • Fig. S2. Restoration of wild-type slpA to the chromosome of FM2.5 and FM2.6.
  • Fig. S3. Avidocin-CD sensitivity correlates with SLCT.
  • Fig. S4. C. difficile strain sensitivity patterns to Avidocin-CDs and Diffocin-4.
  • Fig. S5. Comparison of C. difficile bacteriophage host range versus Avidocin-CD sensitivity.
  • Fig. S6. Clustal Omega alignment of SlpA sequences from strains 630 and M68.
  • Fig. S7. Characterization of sporulation.
  • Fig. S8. Spore morphology and thermal sensitivity.
  • Fig. S9. Expression of toxin B in vitro.
  • Fig. S10. Schematic diagram summarizing the roles of S-layer in C. difficile biology and pathophysiology.
  • Fig. S11. Schematic describing Avidocin-CD construction.
  • Table S1. Newly identified C. difficile phages.
  • Table S2. Primers used in this study.
  • Table S3. GenBank accession identifiers.
  • Legend for table S4
  • Reference (42)

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Table S4 (Microsoft Excel format). Primary data.

[Download Table S4]