Supplementary Materials

Supplementary Material for:

Direct detection of early-stage cancers using circulating tumor DNA

Jillian Phallen, Mark Sausen, Vilmos Adleff, Alessandro Leal, Carolyn Hruban, James White, Valsamo Anagnostou, Jacob Fiksel, Stephen Cristiano, Eniko Papp, Savannah Speir, Thomas Reinert, Mai-Britt Worm Orntoft, Brian D. Woodward, Derek Murphy, Sonya Parpart-Li, David Riley, Monica Nesselbush, Naomi Sengamalay, Andrew Georgiadis, Qing Kay Li, Mogens Rørbæk Madsen, Frank Viborg Mortensen, Joost Huiskens, Cornelis Punt, Nicole van Grieken, Remond Fijneman, Gerrit Meijer, Hatim Husain, Robert B. Scharpf, Luis A. Diaz, Siân Jones, Sam Angiuoli, Torben Ørntoft, Hans Jørgen Nielsen, Claus Lindbjerg Andersen, Victor E. Velculescu*

*Corresponding author. Email: velculescu{at}jhmi.edu

Published 16 August 2017, Sci. Transl. Med. 9, eaan2415 (2017)
DOI: 10.1126/scitranslmed.aan2415

This PDF file includes:

  • Fig. S1. Simulations using limited exogenous barcodes.
  • Fig. S2. Validation of the TEC-Seq approach.
  • Fig. S3. Mutation frequencies in cancer genes.
  • Fig. S4. ctDNA mutant allele fractions in serial blood draws.
  • Fig. S5. Comparison of ctDNA mutant allele fractions measured by TEC-Seq and ddPCR.
  • Fig. S6. ctDNA and tumor heterogeneity.
  • Fig. S7. Preoperative ctDNA mutant allele fractions in colorectal cancer patients.
  • Fig. S8. Preoperative CEA in colorectal cancer patients.
  • Fig. S9. Conversion efficiency of cfDNA.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1. Genes analyzed by TEC-Seq (provided in a separate Excel file).
  • Table S2. Summary of TEC-Seq validation (provided in a separate Excel file).
  • Table S3. Summary of patients analyzed (provided in a separate Excel file).
  • Table S4. Summary of genomic analyses (provided in a separate Excel file).
  • Table S5. Alterations in blood cell proliferation genes in healthy individuals and cancer patients (provided in a separate Excel file).
  • Table S6. Germline alterations identified in cfDNA (provided in a separate Excel file).
  • Table S7. Somatic alterations detected in cfDNA of cancer patients (provided in a separate Excel file).
  • Table S8. Summary of colorectal cancer patient outcomes (provided in a separate Excel file).

[Download Tables S1 to S8]