Supplementary Materials

Supplementary Material for:

FolamiRs: Ligand-targeted, vehicle-free delivery of microRNAs for the treatment of cancer

Esteban A. Orellana, Srinivasarao Tenneti, Loganathan Rangasamy, L. Tiffany Lyle, Philip S. Low, Andrea L. Kasinski*

*Corresponding author. Email: akasinski{at}purdue.edu

Published 2 August 2017, Sci. Transl. Med. 9, eaam9327 (2017)
DOI: 10.1126/scitranslmed.aam9327

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Fol-DBCO ligand synthesis and LC-MS spectral analysis.
  • Fig. S2. Evaluation of folate-miRNA conjugation measured by 15% native TAE PAGE.
  • Fig. S3. MALDI spectra of FolamiRs.
  • Fig. S4. NIR-folate ligand synthesis and LC-MS spectral analysis.
  • Fig. S5. Evaluation of NIR-FolamiR conjugation measured by 15% native TAE PAGE.
  • Fig. S6. MALDI spectra of NIR-FolamiR conjugates.
  • Fig. S7. miR-34a Renilla luciferase sensor response to miR-34a transfection.
  • Fig. S8. Evaluation of MB-231 miR-34a sensor cells.
  • Fig. S9. Folate-mediated delivery of functional siLuc2.
  • Fig. S10. Serum stability of FolamiRs and duplex RNA oligos.
  • Fig. S11. In vivo tumor growth response to dose titration of FolamiR-34a.
  • Fig. S12. miR-34a copy number in tumors treated with FolamiR-34a in mice.
  • Fig. S13. Serum cytokines and maximum tolerated FolamiR dose study in mice.
  • References (5964)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Individual-level data.