Supplementary Materials

Supplementary Material for:

A phenotypically and functionally distinct human TH2 cell subpopulation is associated with allergic disorders

Erik Wambre,* Veronique Bajzik, Jonathan H. DeLong, Kimberly O'Brien, Quynh-Anh Nguyen, Cate Speake, Vivian H. Gersuk, Hannah A. DeBerg, Elizabeth Whalen, Chester Ni, Mary Farrington, David Jeong, David Robinson, Peter S. Linsley, Brian P. Vickery, William W. Kwok

*Corresponding author. Email: ewambre{at}benaroyaresearch.org

Published 2 August 2017, Sci. Transl. Med. 9, eaam9171 (2017)
DOI: 10.1126/scitranslmed.aam9171

This PDF file includes:

  • Fig. S1. Flow cytometric plots showing phenotyping of ex vivo enriched allergen-specific CD4+ T cells in allergic subjects.
  • Fig. S2. Characteristics of allergic disease causing CD4+ T cells.
  • Fig. S3. Allergen-specific TH2 cells are highly mature cells.
  • Fig. S4. Allergen-specific TH2 cells fall into the CD27CD161+CD45RBCD49d+CD4+ T cell subset.
  • Fig. S5. Discrimination between proallergic TH2A and conventional TH2 cell subset.
  • Fig. S6. Influence of OFC and oral immunotherapy on peanut-specific CD4+ T cells.
  • Fig. S7. Expression of TH2 cytokines is restricted to the allergen-specific TH2A cell subset.
  • Fig. S8. Overview of TH2A phenotype.
  • Fig. S9. Gating strategy for TH cell subset isolation.
  • Table S1. List of antibodies used in this study for the allergen-specific CD4+ T cell profiling.
  • Table S2. List of all up-regulated genes in the TH2A cell subset relative to conventional TH2 cells.
  • Legend for table S3
  • Table S4. List of pMHCII tetramer reagents used in this study.

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Other Supplementary Material for this manuscript includes the following:

  • Table S3 (Microsoft Excel format). Primary data.