Supplementary Materials

Supplementary Material for:

The microvascular niche instructs T cells in large vessel vasculitis via the VEGF-Jagged1-Notch pathway

Zhenke Wen, Yi Shen, Gerald Berry, Farhad Shahram, Yinyin Li, Ryu Watanabe, Yaping Joyce Liao, Jörg J. Goronzy, Cornelia M. Weyand*

*Corresponding author. Email: cweyand{at}stanford.edu

Published 19 July 2017, Sci. Transl. Med. 9, eaal3322 (2017)
DOI: 10.1126/scitranslmed.aal3322

This PDF file includes:

  • Fig. S1. IL-6 but not VEGF drives endothelial Delta-like 1 expression.
  • Fig. S2. Activation of ECs in response to plasma treatment.
  • Fig. S3. Infrequent expression of Notch1 on CD8 T cells.
  • Fig. S4. Low expression of Notch4 on CD4 T cells.
  • Fig. S5. Lack of memory CD4 T cell expansion in GCA.
  • Fig. S6. Kinetics and durability of Jagged1 induction in ECs.
  • Fig. S7. Plasma-pretreated HUVECs induce effector T cells.
  • Fig. S8. IL-6–stimulated ECs fail to induce effector T cells.
  • Fig. S9. VEGF fails to inhibit the activation of human CD4 T cells.
  • Fig. S10. Efficiency of Notch1 knockdown by siRNA transfection.
  • Fig. S11. Induction of T effector cells by plasma-treated HUVECs is Jagged1-dependent.
  • Fig. S12. RA plasma fails to induce T cell stimulatory capacity of ECs.
  • Fig. S13. Notch1 receptor expression on CD4 T cells.
  • Fig. S14. T cell differentiation induced by endothelial Jagged1 is mTORC1-dependent.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Individual subject–level data.

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