Supplementary Materials

Supplementary Material for:

Detection of early pancreatic ductal adenocarcinoma with thrombospondin-2 and CA19-9 blood markers

Jungsun Kim, William R. Bamlet, Ann L. Oberg, Kari G. Chaffee, Greg Donahue, Xing-Jun Cao, Suresh Chari, Benjamin A. Garcia, Gloria M. Petersen,* Kenneth S. Zaret*

*Corresponding author. Email: zaret{at}upenn.edu

Published 12 July 2017, Sci. Transl. Med. 9, eaah5583 (2017)
DOI: 10.1126/scitranslmed.aah5583

This PDF file includes:

  • Fig. S1. Validation of cross-reactivity of antibodies enclosed in THBS2 ELISA with THBS1 by Western blot.
  • Fig. S2. Validation of cross-reactivity and interference of THBS1 in THBS2 ELISA.
  • Fig. S3. Reproducibility of the ELISA for THBS2.
  • Fig. S4. Distribution of THBS2 values in phase 2 samples.
  • Fig. S5. Cross-validation tests, performed 1 year apart, of THBS2 concentrations in the same set of plasmas as determined in different laboratories.
  • Fig. S6. Representative immunohistochemistry images of THBS2 in human normal pancreas, pancreatitis, and PDAC tissue.
  • Table S1. List of 53 proteins secreted or released from 10-22 cell, PanIN-stage lesions that are at low abundance in healthy human plasma proteome and RNA-seq databases.
  • Table S2. Mass spectrometry assessment of THBS2 concentrations in phase 1 plasma samples.
  • Table S3. Range and median values of THBS2 and CA19-9 in this study.
  • Table S4. Impact of excluding stage IIB (and unspecified stage II) subjects.
  • Table S5A. THBS2 values by sex and diabetes mellitus status.
  • Table S5B. Spearman correlation analysis of age and THBS2 values.
  • Table S6A. Obstructive jaundice cases in the PDAC cohorts.
  • Table S6B. THBS2 and CA19-9 values and obstructive jaundice status.
  • Table S6C. AUC values for CA19-9, THBS2, and combined markers by jaundice status in phases 2a and 2b of PDAC cases versus controls.
  • Table S7A. Cross-tabulation of normal versus elevated THBS2 values, given a cutoff of 42 ng/ml, for the original cross-validation THBS2 assays (κ = 0.786).
  • Table S7B. Cross-tabulation of normal versus elevated scaled THBS2 values, given a cutoff of 2.47, for the original and cross-validation THBS2 assays (κ = 0.895).
  • Table S8. Summary of THBS2 immunohistochemistry in a total of 42 human PDAC and 4 cases of incidental PanIN and intraductal papillary mucinous neoplasm by immunohistochemistry.

[Download PDF]