Supplementary Materials

Supplementary Material for:

Neoadjuvant chemotherapy induces breast cancer metastasis through a TMEM-mediated mechanism

George S. Karagiannis,* Jessica M. Pastoriza, Yarong Wang, Allison S. Harney, David Entenberg, Jeanine Pignatelli, Ved P. Sharma, Emily A. Xue, Esther Cheng, Timothy M. D'Alfonso, Joan G. Jones, Jesus Anampa, Thomas E. Rohan, Joseph A. Sparano, John S. Condeelis,* Maja H. Oktay*

*Corresponding author. Email: georgios.karagiannis{at} (G.S.K.); john.condeelis{at} (J.S.C.); maja.oktay{at} (M.H.O.)

Published 5 July 2017, Sci. Transl. Med. 9, eaan0026 (2017)
DOI: 10.1126/scitranslmed.aan0026

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Histological sections of control and paclitaxel-treated mice.
  • Fig. S2. Macrophage population dynamics in control and paclitaxel-treated mice.
  • Fig. S3. Microvascular density in control and paclitaxel-treated mice.
  • Fig. S4. IVI and extravascular dextran intensity analysis.
  • Fig. S5. Experimental design of in vivo metastasis dissemination assays.
  • Fig. S6. Frequency of bursting normalized to TMEM sites.
  • Fig. S7. MENAINV expression at the gene and protein levels.
  • Fig. S8. The association of Mena isoform expression with TIE2hi/VEGFhi macrophages.
  • Fig. S9. Macrophage population dynamics after paclitaxel treatment in MENA−/− mice.
  • Fig. S10. Correlation of TMEM scoring between pathologists.
  • Table S1. Forward and reverse primer sequences used to identify transmission of the disrupted Mena allele in MENA−/− mice.
  • Legends for videos S1 to S3
  • References (5565)

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Video S1 (.avi format). Bursting close to a TMEM site of a paclitaxel-treated mouse.
  • Video S2 (.avi format). Bursting close to a TMEM site of a second paclitaxel-treated mouse.
  • Video S3 (.avi format). Absence of bursting in the vasculature of a paclitaxel-treated mouse.