Supplementary Materials

Supplementary Material for:

Donor pulmonary intravascular nonclassical monocytes recruit recipient neutrophils and mediate primary lung allograft dysfunction

Zhikun Zheng, Stephen Chiu, Mahzad Akbarpour, Haiying Sun, Paul A. Reyfman, Kishore R. Anekalla, Hiam Abdala-Valencia, Daphne Edgren, Wenjun Li, Daniel Kreisel, Farida V. Korobova, Ramiro Fernandez, Alexandra McQuattie-Pimentel, Zheng J. Zhang, Harris Perlman, Alexander V. Misharin, G. R. Scott Budinger, Ankit Bharat*

*Corresponding author. Email: abharat{at}

Published 14 June 2017, Sci. Transl. Med. 9, eaal4508 (2017)
DOI: 10.1126/scitranslmed.aal4508

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Cytokine analysis of the lung allograft.
  • Fig. S2. Representative gating strategy to evaluate the myeloid cell populations in the lungs.
  • Fig. S3. Perfusion of the heart-lung block.
  • Fig. S4. Effects of monocyte depletion strategies on the donor.
  • Fig. S5. Effects of donor clo-lip treatment on the 24-hour posttransplant allograft.
  • Fig. S6. Donor clo-lip treatment abrogates neutrophil influx after syngeneic lung transplant.
  • Fig. S7. Effects of CX3CR1 deletion on nonmonocyte cell populations in the lung and blood and on the posttransplant allograft.
  • Fig. S8. Effects of NR4A1 deletion on non-monocyte cell populations in the lung and blood and on the posttransplant allograft.
  • Fig. S9. All neutrophils were of recipient origin after 24 hours of reperfusion in the allograft.
  • Fig. S10. Representative gating strategy of GFP-expressing cells in the lungs of Cx3cr1gfp/+ and Cx3cr1gfp/gfp mice.
  • Fig. S11. NCMs do not differentiate upon lipopolysaccharide challenge.
  • Fig. S12. Immunofluorescence microscopy of human lung biopsy from a patient experiencing PGD.
  • Fig. S13. Schematic to illustrate the role of pulmonary intravascular NCMs in mediating neutrophil infiltration and lung allograft injury.
  • Legend for table S1
  • Legends for movies S1 and S2
  • References (5257)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Primary data.
  • Movie S1 (.mov format). PBS-lip–treated control mice reveal profound neutrophil infiltration into the lung allograft after reperfusion.
  • Movie S2 (.mov format). Intravenous clo-lip treatment of the donors abrogates neutrophil infiltration into the allograft after reperfusion.

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[Download Movies S1 and S2]