Supplementary Materials

Supplementary Material for:

Vaccine priming is restricted to draining lymph nodes and controlled by adjuvant-mediated antigen uptake

Frank Liang, Gustaf Lindgren, Kerrie J. Sandgren, Elizabeth A. Thompson, Joseph R. Francica, Anja Seubert, Ennio De Gregorio, Susan Barnett, Derek T. O'Hagan, Nancy J. Sullivan, Richard A. Koup, Robert A. Seder, Karin Loré*

*Corresponding author. Email: karin.lore{at}

Published 7 June 2017, Sci. Transl. Med. 9, eaal2094 (2017)
DOI: 10.1126/scitranslmed.aal2094

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Accumulation of neutrophils in response to adjuvant injection.
  • Fig. S2. MxA expression after alum or MF59 immunization.
  • Fig. S3. Env uptake by Env-naïve or Env-boosted animals.
  • Fig. S4. In situ analysis of GCs.
  • Fig. S5. Schematic overview on adjuvant-induced innate and adaptive immune responses.
  • Table S1. Intramuscular immunization schema.
  • Table S2. Abs for flow cytometry.
  • References (41, 42)

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Other Supplementary Material for this manuscript includes the following:

  • Table S3 (Microsoft Excel format). Primary data.

[Download Table S3]