Supplementary Materials

Supplementary Material for:

Radiation therapy primes tumors for nanotherapeutic delivery via macrophage-mediated vascular bursts

Miles A. Miller, Ravi Chandra, Michael F. Cuccarese, Christina Pfirschke, Camilla Engblom, Shawn Stapleton, Utsarga Adhikary, Rainer H. Kohler, James F. Mohan, Mikael J. Pittet, Ralph Weissleder*

*Corresponding author. Email: rweissleder{at}mgh.harvard.edu

Published 31 May 2017, Sci. Transl. Med. 9, eaal0225 (2017)
DOI: 10.1126/scitranslmed.aal0225

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Validation of computational segmentation algorithms.
  • Fig. S2. Overview of irradiation and nanoformulation properties.
  • Fig. S3. IVM and TEM examples.
  • Fig. S4. Dose-dependent RT effects.
  • Fig. S5. RT-enhanced TNP delivery requires 3-day dose staggering and drug nanoencapsulation and occurs through direct and redistribution cellular uptake mechanisms.
  • Fig. S6. Imaging colocalization of nanoformulations in tumor phagocytes.
  • Fig. S7. Modeling heterogeneous permeability.
  • Fig. S8. Clodronate liposomes and cyclophosphamide block and enhance vascular bursting, respectively.
  • Fig. S9. Illustration of TAM-mediated vascular bursting.
  • Table S1. List of biophysical transport properties used to simulate vascular bursts.
  • Legend for movie S1
  • References (3640)

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Other Supplementary Material for this manuscript includes the following:

  • Table S2 (Microsoft Excel format). Individual-level data.
  • Movie S1 (.avi format). Heterogeneous dextran extravasation in irradiated tumors.

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[Download Movie S1]