Supplementary Materials

Supplementary Material for:

Complement C3 deficiency protects against neurodegeneration in aged plaque-rich APP/PS1 mice

Qiaoqiao Shi, Saba Chowdhury, Rong Ma, Kevin X. Le, Soyon Hong, Barbara J. Caldarone, Beth Stevens, Cynthia A. Lemere*

*Corresponding author. Email: clemere{at}

Published 31 May 2017, Sci. Transl. Med. 9, eaaf6295 (2017)
DOI: 10.1126/scitranslmed.aaf6295

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Behavioral testing of locomotion and anxiety.
  • Fig. S2. C3 deficiency had no effect on Aβ deposition in young APP/PS1 mice at the earliest stage of plaque deposition.
  • Fig. S3. Immunoreactivity of Aβx–42, Aβx–40, and Aβ1–x in aged mice.
  • Fig. S4. Glial immunoreactivity was similar in young WT, APP/PS1, and APP/PS1;C3 KO mice at the earliest stage of plaque deposition.
  • Fig. S5. C3 deficiency resulted in morphological differences in Iba-1–immunoreactive cells in APP/PS1 aged mice.
  • Fig. S6. C3 deficiency resulted in reduced activation of microglia within Aβ plaques in aged APP/PS1 mice but not in aged J20 mice.
  • Fig. S7. MAP-2–positive dendrites were better preserved within Aβ plaques in 16-month-old C3-deficient APP/PS1 mice versus APP/PS1 mice.
  • Fig. S8. The amounts of APP and PS1 were not significantly different between APP/PS1 and APP/PS1;C3 KO mice.
  • References (68, 69)

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