Supplementary Materials

Supplementary Material for:

The Src/c-Abl pathway is a potential therapeutic target in amyotrophic lateral sclerosis

Keiko Imamura, Yuishin Izumi, Akira Watanabe, Kayoko Tsukita, Knut Woltjen, Takuya Yamamoto, Akitsu Hotta, Takayuki Kondo, Shiho Kitaoka, Akira Ohta, Akito Tanaka, Dai Watanabe, Mitsuya Morita, Hiroshi Takuma, Akira Tamaoka, Tilo Kunath, Selina Wray, Hirokazu Furuya, Takumi Era, Kouki Makioka, Koichi Okamoto, Takao Fujisawa, Hideki Nishitoh, Kengo Homma, Hidenori Ichijo, Jean-Pierre Julien, Nanako Obata, Masato Hosokawa, Haruhiko Akiyama, Satoshi Kaneko, Takashi Ayaki, Hidefumi Ito, Ryuji Kaji, Ryosuke Takahashi, Shinya Yamanaka, Haruhisa Inoue*

*Corresponding author. Email: haruhisa{at}cira.kyoto-u.ac.jp

Published 24 May 2017, Sci. Transl. Med. 9, eaaf3962 (2017)
DOI: 10.1126/scitranslmed.aaf3962

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Generation of iPSCs from control and ALS patients.
  • Fig. S2. Characterization of motor neurons and genetic correction of mutant SOD1 iPSCs.
  • Fig. S3. Investigation of the effects of Src/c-Abl inhibitors.
  • Fig. S4. mRNA expression changes after bosutinib treatment by single-cell analysis.
  • Fig. S5. Decrease in misfolded proteins after bosutinib treatment.
  • Fig. S6. Analysis of postmortem ALS spinal cord tissue.
  • Table S1. List of iPSC clones.
  • Table S2. Sequence variations in exon regions for sporadic ALS.
  • Table S3. List of hit compounds.
  • Table S4. Genes highly expressed in mutant SOD1 ALS motor neurons identified by single-cell RNA-seq.
  • Table S5. Genes highly expressed in control motor neurons identified by single-cell RNA-seq.
  • Table S6. List of postmortem spinal cord tissue for immunohistochemistry.
  • Table S7. List of postmortem spinal cord tissue for ELISA.
  • Table S8. Primer list for editing of SOD1 gene.
  • Table S9. Primer list for quantitative PCR.
  • References (4856)

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