Supplementary Materials

Supplementary Material for:

Rapid and specific detection of Asian- and African-lineage Zika viruses

Nunya Chotiwan, Connie D. Brewster, Tereza Magalhaes, James Weger-Lucarelli, Nisha K. Duggal, Claudia Rückert, Chilinh Nguyen, Selene M. Garcia Luna, Joseph R. Fauver, Barb Andre, Meg Gray, William C. Black IV, Rebekah C. Kading, Gregory D. Ebel, Guillermina Kuan, Angel Balmaseda, Thomas Jaenisch, Ernesto T. A. Marques, Aaron C. Brault, Eva Harris, Brian D. Foy, Sandra L. Quackenbush, Rushika Perera, Joel Rovnak*

*Corresponding author. Email: joel.rovnak{at}colostate.edu

Published 3 May 2017, Sci. Transl. Med. 9, eaag0538 (2017)
DOI: 10.1126/scitranslmed.aag0538

This PDF file includes:

  • Fig. S1. Confirmation of Zika-specific, RNA-dependent LAMP amplification.
  • Fig. S2. Control samples containing each virus RNA.
  • Fig. S3. Alignment of Zika LAMP primer sequences with target sequences in all known Zika virus sequences.
  • Fig. S4. Direct detection of Zika virus in mosquitoes.
  • Fig. S5. Confirmation of Zika-specific LAMP amplification from mosquito homogenate.
  • Fig. S6. Zika LAMP assay is dependent upon RNA.
  • Fig. S7. Visual LAMP assay of mosquito homogenates.
  • Fig. S8. Limits of PRVABC59-LAMP detection match RT-PCR.
  • Fig. S9. LAMP assay detects PRVABC59 virus in human biofluids.
  • Table S1. Zika LAMP primers.
  • Table S2. Plaque titration of split, infected mosquitoes.
  • Table S3. Plaque titration of midguts, salivary glands, and carcass.
  • Table S4A. LAMP assay LOD for genome copies.
  • Table S4B. TaqMan LOD for genome copies.
  • Table S5A. Plasma 1:1000 LOD.
  • Table S5B. Plasma 1:1000 turbidity LOD.
  • Table S6. Blood 1:1000 LOD.
  • Table S7. Urine 1:1000 LOD.
  • Table S8. Saliva 1:1000 LOD.
  • Table S9. Semen 1:1000 LOD.
  • Table S10. Nicaraguan serum and RNA samples.
  • Table S11. Brazilian plasma and RNA samples.

[Download PDF]