Supplementary Materials

Supplementary Material for:

Smartphone-controlled optogenetically engineered cells enable semiautomatic glucose homeostasis in diabetic mice

Jiawei Shao, Shuai Xue, Guiling Yu, Yuanhuan Yu, Xueping Yang, Yu Bai, Sucheng Zhu, Linfeng Yang, Jianli Yin, Yidan Wang, Shuyong Liao, Sanwei Guo, Mingqi Xie, Martin Fussenegger, Haifeng Ye*

*Corresponding author. Email: hfye{at}

Published 26 April 2017, Sci. Transl. Med. 9, eaal2298 (2017)
DOI: 10.1126/scitranslmed.aal2298

This PDF file includes:

  • Materials and Methods
  • Fig. S1. ECNU-TeleMed app remotely controlling the SmartController system.
  • Fig. S2. Blue light–triggered optogenetic devices.
  • Fig. S3. Optimization of the FRL-v1 system.
  • Fig. S4. FRL-triggered optogenetic devices.
  • Fig. S5. FRL-v1 control experiments.
  • Fig. S6. Fluorescence micrographs profiling EGFP expression kinetics of FRL-v1.
  • Fig. S7. The orthogonality of the FRL-v1 system.
  • Fig. S8. Optimization of the FRL-v2 system.
  • Fig. S9. The orthogonality of the FRL-v2 system.
  • Fig. S10. Fluorescence micrographs profiling EGFP expression kinetics of FRL-v2.
  • Fig. S11. Spatial control of FRL-dependent transgene expression.
  • Fig. S12. Impact of ectopic FRL-v2 constituents’ expression on the metabolic integrity of human cells.
  • Fig. S13. FRL-v2–dependent SEAP expression in different types of implants.
  • Fig. S14. FRL-v2 triggered insulin and EGFP expression in vitro.
  • Fig. S15. FRL-v2 triggered shGLP-1 expression in vitro.
  • Fig. S16. Impact of FRL on glucose and insulin metabolism of db/db mice.
  • Fig. S17. Unwanted activation of the FRL-v2 system by white light.
  • Fig. S18. Photographs of the custom-designed EEC.
  • Fig. S19. Implementation of the SmartController 2.0 system described in Fig. 5.
  • Fig. S20. Characteristics of HydrogeLED.
  • Fig. S21. FRL-v2–dependent SEAP expression in custom-designed HydrogeLED implants.
  • Fig. S22. Implementation of the SmartController 3.0 system described in Fig. 6.
  • Fig. S23. Different GTs programmed to translate into corresponding FRL illumination strengths.
  • Fig. S24. Schematic of the circuit design of a 4 × 6 FRL LED array.
  • Fig. S25. Photograph of the custom-designed FRL LED array and the physiotherapy lamp.
  • Table S1. Plasmids designed and used in this study.
  • References (5056)

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Other Supplementary Material for this manuscript includes the following:

  • Table S2. Individual-level data (provided in Excel).
  • Table S3. P values (provided in Excel).

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