Supplementary Materials

Supplementary Material for:

Glycosaminoglycan-based hydrogels capture inflammatory chemokines and rescue defective wound healing in mice

Nadine Lohmann, Lucas Schirmer, Passant Atallah, Elke Wandel, Ruben A. Ferrer, Carsten Werner, Jan C. Simon, Sandra Franz,* Uwe Freudenberg*

*Corresponding author. Email: sandra.franz{at}medizin.uni-leipzig.de (S.F.); freudenberg{at}ipfdd.de (U.F.)

Published 19 April 2017, Sci. Transl. Med. 9, eaai9044 (2017)
DOI: 10.1126/scitranslmed.aai9044

This PDF file includes:

  • Supplemental experimental procedures
  • Fig. S1. Characterization of conditioned medium from activated primary human inflammatory macrophages and dermal fibroblasts.
  • Fig. S2. Transmigration assays with monocytes and PMNs using conditioned medium from activated dermal fibroblasts.
  • Fig. S3. Characterization of scavenging effects of starPEG-GAG hydrogels with recombinant human MCP-1 and IL-8.
  • Fig. S4. Gating strategy used to identify PMNs and monocytes present in wound tissue.
  • Fig. S5. Histological characterization of starPEG-GAG hydrogels applied onto wounds.
  • Fig. S6. Modulation of cytokine protein concentrations in wounds by starPEG-GAG hydrogels.
  • Fig. S7. Scavenging and pro-wound healing effects of starPEG-GAG hydrogels in wounds infected with S. aureus.
  • Fig. S8. Characterization of scavenging effects of starPEG-GAG hydrogels in human wound fluids.
  • Table S1. Characterization of scavenging effects of starPEG-GAG hydrogels in human wound fluids.
  • Table S2. Chemokine concentration and infection status of patient samples.
  • Table S3. Primer sequences and annealing temperatures (Tanneal) used for quantitative polymerase chain reaction.

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Other Supplementary Material for this manuscript includes the following:

  • Table S4 (Microsoft Excel format). Individual level data corresponding to the different figures.

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