Supplementary Materials

Supplementary Material for:

An automated smartphone-based diagnostic assay for point-of-care semen analysis

Manoj Kumar Kanakasabapathy, Magesh Sadasivam, Anupriya Singh, Collin Preston, Prudhvi Thirumalaraju, Maanasa Venkataraman, Charles L. Bormann, Mohamed Shehata Draz, John C. Petrozza, Hadi Shafiee*

*Corresponding author. Email: hshafiee{at}bwh.harvard.edu

Published 22 March 2017, Sci. Transl. Med. 9, eaai7863 (2017)
DOI: 10.1126/scitranslmed.aai7863

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Image of a micrometer scale taken with the smartphone-based semen analyzer.
  • Fig. S2. Images of microbeads and sperm taken with a laboratory-based microscope and the smartphone-based semen analyzer.
  • Fig. S3. Process flow of microchip fabrication.
  • Fig. S4. Smartphone application flow.
  • Fig. S5. Device performance for samples with concentrations below 100 million sperm/ml.
  • Fig. S6. Bland-Altman analysis for the measurement of sperm curvilinear (VCL) and linear velocities (VSL).
  • Fig. S7. Weight scale schematic and design.
  • Fig. S8. Weight scale performance in estimating the total count and total motile count.
  • Fig. S9. Bland-Altman analysis of the three phones.
  • Fig. S10. Overall agreement and diagnostic performance of the smartphone-based semen analyzer.
  • Fig. S11. Steps of operation for semen testing using the smartphone-based semen analyzer.
  • Table S1. Table of costs.
  • Table S2. Processing times for the 350 samples tested by trained and untrained users.
  • Table S3. Raw data of cryopreserved samples collected for measuring sperm concentration.
  • Table S4. Raw data collected from clinical specimens at MGH fertility clinic tested by trained users.
  • Table S5. Repeatability measures for the concentration parameter.
  • Table S6. Repeatability measures for the motility parameter.
  • Table S7. Diagnostic results for samples tested by trained users.
  • Table S8. Comparison of the performance of three different phones with the smartphone-based semen analyzer.
  • Table S9. Raw data collected from clinical specimens at MGH fertility clinic tested by untrained users.
  • Table S10. Diagnostic results for samples tested by untrained users.
  • Table S11. CASA triplicate measurements at MGH fertility clinic for 112 samples.
  • Table S12. Measurement of CASA accuracy against manual analysis.
  • Legend for movie S1

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Video of a semen sample recorded with the smartphone-based semen analyzer.