Supplementary Materials

Supplementary Material for:

Bacterial virulence phenotypes of Escherichia coli and host susceptibility determine risk for urinary tract infections

Henry L. Schreiber IV, Matt S. Conover, Wen-Chi Chou, Michael E. Hibbing, Abigail L. Manson, Karen W. Dodson, Thomas J. Hannan, Pacita L. Roberts, Ann E. Stapleton, Thomas M. Hooton, Jonathan Livny,* Ashlee M. Earl,* Scott J. Hultgren*

*Corresponding author. Email: livny{at}broadinstitute.org (J.L.); aearl{at}broadinstitute.org (A.M.E.); hultgren{at}wusm.wustl.edu (S.J.H.)

Published 22 March 2017, Sci. Transl. Med. 9, eaaf1283 (2017)
DOI: 10.1126/scitranslmed.aaf1283

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Sample collection and patient time line.
  • Fig. S2. Measurement of UAEC gene carriage and nucleotide diversity in core genome.
  • Fig. S3. Kidney colonization by B2 and non-B2 UAEC strains.
  • Fig. S4. Gut-associated E. coli are poor colonizers in the C3H/HeN mouse model of UTI.
  • Fig. S5. IBC formation by B2 and non-B2 UAEC strains.
  • Fig. S6. Carriage of PAI IIUTI89 enhances competitive fitness in chronic UTI in C3H/HeN mice.
  • Fig. S7. Hemagglutination is correlated to colonization efficiency in both B2 and non-B2 UAEC strains.
  • Fig. S8. Differentially expressed genes in UAEC relative to UTI89.
  • Fig. S9. Expression of core genes under type 1 pili–inducing culture conditions correlates with bladder colonization in UAEC.
  • Fig. S10. Motility does not correlate with colonization efficiency in either B2 or non-B2 UAEC strains.
  • Fig. S11. Both B2 and non-B2 UAEC strains are capable of forming persistent bladder reservoirs in C57BL/6 mice.
  • Table S1. Clinical information of enrolled patients.
  • Table S2. Characteristics of UAEC isolates.
  • Table S3. Reference E. coli strains.
  • Table S4. List of PUFs.
  • Table S5. Gut-associated E. coli strain characteristics.
  • Table S6. IBC formation in select UAEC strains at 6 hpi in C3H/HeN mice.
  • Table S7. Presence of brnAT genes in robust and deficient colonizer UAEC strains.
  • Table S8. Reads mapped to core genome of UAEC strains.
  • Legend for table S9
  • Table S10. Expression of PUF genes in select UAEC strains.
  • Legends for tables S11 and S12
  • Table S13. UAEC strain clinical data.
  • Table S14. UAEC strain sequencing and genome assembly results.
  • References (8286)

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Other Supplementary Material for this manuscript includes the following:

  • Table S9 (Microsoft Excel format). DESeq analysis of UAEC transcription of core genome.
  • Table S11 (Microsoft Excel format). Linear correlation of core gene expression with bladder colonization.
  • Table S12 (Microsoft Excel format). Functions enriched in genes associated with bladder colonization.

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