Supplementary Materials

Supplementary Material for:

Antagonism of EGFR and Notch limits resistance to EGFR inhibitors and radiation by decreasing tumor-initiating cell frequency

Shi Hu,* Wenyan Fu, Tian Li, Qingning Yuan, Feifei Wang, Gaojian Lv, Yuanyuan Lv, Xiaoyan Fan, Yafeng Shen, Fangxing Lin, Ying Tang, Xuting Ye, Yongji Yang, Changhai Lei*

*Corresponding author. Email:, hus{at} (S.H.); lei{at} (C.L.)

Published 8 March 2017, Sci. Transl. Med. 9, eaag0339 (2017)
DOI: 10.1126/scitranslmed.aag0339

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Different response to EGFR blockade in different cell subpopulations.
  • Fig. S2. Cellular plasticity of CSC subpopulations with or without EGFR blockade.
  • Fig. S3. Different radiation-induced EGFR signaling activation of CSC subpopulations.
  • Fig. S4. Notch receptor expression in different cell subpopulations.
  • Fig. S5. siRNA-mediated Notch receptor down-regulation.
  • Fig. S6. Design and characterization of dual-targeting antibody CT16.
  • Fig. S7. Body weight change over time in response to different drugs.
  • Fig. S8. Different effects of drugs on the CD133+ALDH cell subpopulation.
  • Fig. S9. Hypothetical models of the effects of different antibodies.
  • Fig. S10. Characterization of progressing tumors.
  • Fig. S11. EGFR signaling after XRT combined with different antibodies.
  • Fig. S12. Notch and EGFR signaling of tumors after treatment with different antibodies.
  • Table S1. Analysis of ALDH activity and CD133 cell surface expression in different cancer cells.
  • Table S2. Sequences of different antibodies used in the study.
  • Table S3. Selected analytical and affinity data for the parental antibodies and CT16.
  • Table S4. Pharmacokinetic parameters of recombinant antibodies in mice.
  • Table S5. Affinity data for different antibodies.
  • Table S6. Tumor growth inhibitory effects of different drugs in murine xenograft models.
  • Table S7. Tumor growth inhibitory effects of different drugs in PDX models.
  • References (5154)

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Other Supplementary Material for this manuscript includes the following:

  • Table S8 (Microsoft Excel format). Exact P values.
  • Table S9 (Microsoft Excel format). Tumor measurements.

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