Supplementary Materials

Supplementary Material for:

Evolutionarily conserved serum microRNAs predict radiation-induced fatality in nonhuman primates

Wojciech Fendler, Beata Malachowska, Khyati Meghani, Panagiotis A. Konstantinopoulos, Chandan Guha,* Vijay K. Singh,* Dipanjan Chowdhury*

*Corresponding author. Email: dipanjan_chowdhury{at} (D.C.); vijay.singh{at} (V.K.S.); cguha{at} (C.G.)

Published 1 March 2017, Sci. Transl. Med. 9, eaal2408 (2017)
DOI: 10.1126/scitranslmed.aal2408

This PDF file includes:

  • Fig. S1. Flowchart of miRNA expression comparisons.
  • Fig. S2. Batch effect removal.
  • Fig. S3. Overlap of radiation-induced changes of miRNA expression in the mouse and macaque experiments.
  • Fig. S4. Performance of the three-miRNA irradiation classifier on vehicle- and GT3-treated samples.
  • Fig. S5. Survival of vehicle- and GT3-treated macaques.
  • Fig. S6. Survival curves for miRNAs retained in the survival prediction model.
  • Fig. S7. Differences of miR-16-2 in data normalized to miR-142/miR-320a average.
  • Fig. S8. Correlation of miRNA expression between the primary and validation groups.
  • Fig. S9. Hierarchical clustering of miRNA expression in the validation group.
  • Fig. S10. Survival curves for miR-30a, miR-126, and miR-16-2 in the validation group.
  • Fig. S11. The five-miRNA diagnostic protocol for irradiation detection and outcome prediction.
  • Legends for tables S1 and S2
  • Table S3. miRNA expression in macaques irradiated with different doses.
  • Legend for table S4
  • Table S5. Numerical parameters of logistic regression models.
  • Table S6. Goodness-of-fit evaluation of the irradiation detection model.
  • Table S7. Promoter sequences of miRNAs with similar patterns of radiation-induced expression changes.
  • Legend for table S8
  • Table S9. Overlap of transcription factor binding sites across the three species.
  • Legends for tables S10 to S17
  • Table S18. Comparisons of miRNA expression depending on outcome.
  • Table S19. Differences of outcome frequencies depending on sex.
  • Legend for table S20
  • Table S21. Differences of radiation-induced miRNA expression in the validation group.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1. Raw expression data from miRNA qPCR profiling (separate .xls file).
  • Table S2. Paired sample miRNA expression comparisons (separate .xls file).
  • Table S4. Independent sample miRNA expression comparisons (separate .xls file).
  • Table S8. Transcription factor binding sites in miRNA promoters of human, macaque, and mouse genomes (separate .xls file).
  • Table S10. Promoter sequences of miR-30a (separate .txt file).
  • Table S11. Promoter sequences of miR-126 (separate .txt file).
  • Table S12. Promoter sequences of miR-375 (separate .txt file).
  • Table S13. Promoter sequences of miR-150 (separate .txt file).
  • Table S14. Promoter sequences of mir-133b (separate .txt file).
  • Table S15. Promoter sequences of miR-133a (separate .txt file).
  • Table S16. Promoter sequences of miR-215 (separate .txt file).
  • Table S17. Validated gene targets of radiation-associated evolutionarily conserved miRNAs (separate .xls file).
  • Table S20. Selection of best normalizer miRNAs (separate .xls file).

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