Supplementary Materials

Supplementary Material for:

Transplantation of fecal microbiota from patients with irritable bowel syndrome alters gut function and behavior in recipient mice

Giada De Palma, Michael D. J. Lynch, Jun Lu, Vi T. Dang, Yikang Deng, Jennifer Jury, Genevieve Umeh, Pedro M. Miranda, Marc Pigrau Pastor, Sacha Sidani, Maria Ines Pinto-Sanchez, Vivek Philip, Peter G. McLean, Moreno-Gabriel Hagelsieb, Michael G. Surette, Gabriela E. Bergonzelli, Elena F. Verdu, Philip Britz-McKibbin, Josh D. Neufeld, Stephen M. Collins, Premysl Bercik*

*Corresponding author. Email: bercikp{at}mcmaster.ca

Published 1 March 2017, Sci. Transl. Med. 9, eaaf6397 (2017)
DOI: 10.1126/scitranslmed.aaf6397

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Microbiota profiles in human donors and recipient gnotobiotic mice.
  • Fig. S2. Behavior of germ-free NIH Swiss mice and mice colonized with microbiota from healthy controls, IBS-D patients, and an SPF NIH Swiss mouse.
  • Fig. S3. Colon cytokine levels are similar in healthy control and IBS microbiota–colonized mice.
  • Fig. S4. Biological networks activated in mice with IBS-D microbiota.
  • Fig. S5. Activation of pattern recognition receptors in mice colonized with IBS-D microbiota.
  • Fig. S6. Activation of glucocorticoid receptor signaling in mice with IBS-D microbiota.
  • Fig. S7. Metabolomic profiles in mouse colonized with IBS and healthy microbiota.
  • Table S1. Seven metabolites identified in serum of gnotobiotic mice based on their S-plot scores and P values.
  • Table S2. Immune gene-related networks altered in mice colonized with fecal microbiota from IBS-D patients.
  • Legends for files S1 and S2
  • References (94–96)

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Other Supplementary Material for this manuscript includes the following:

  • File S1. AXIOME script for analysis of Illumina sequence data for healthy and IBS patient fecal samples and corresponding mouse cohort cecal samples.
  • File S2. AXIOME script for analysis of Illumina sequence data for single healthy and IBS patient fecal samples and corresponding mouse cohort fecal and cecal samples.

[Download Files S1 and S2]