Supplementary Materials

Supplementary Material for:

Antimicrobials from human skin commensal bacteria protect against Staphylococcus aureus and are deficient in atopic dermatitis

Teruaki Nakatsuji, Tiffany H. Chen, Saisindhu Narala, Kimberly A. Chun, Aimee M. Two, Tong Yun, Faiza Shafiq, Paul F. Kotol, Amina Bouslimani, Alexey V. Melnik, Haythem Latif, Ji-Nu Kim, Alexandre Lockhart, Keli Artis, Gloria David, Patricia Taylor, Joanne Streib, Pieter C. Dorrestein, Alex Grier, Steven R. Gill, Karsten Zengler, Tissa R. Hata, Donald Y. M. Leung, Richard L. Gallo*

*Corresponding author. Email: rgallo{at}ucsd.edu

Published 22 February 2017, Sci. Transl. Med. 9, eaah4680 (2017)
DOI: 10.1126/scitranslmed.aah4680

This PDF file includes:

  • Methods
  • Fig. S1. Analysis of the skin bacterial community by 16S rRNA gene sequencing and its relationship to the capacity to culture S. aureus from the skin.
  • Fig. S2. Abundance of live Staphylococcus and Staphylococcus-specific DNA on the skin of non-AD and AD subjects.
  • Fig. S3. Specificity of Staphylococcus genus–specific primers for real-time qPCR.
  • Fig. S4. Anti–S. aureus activity in CoNS is stable over time.
  • Fig. S5. Effect of vehicle treatment on the survival of S. aureus on ex situ pigskin or live mouse skin.
  • Fig. S6. MALDI-TOF MS analysis for two AMPs purified from S. hominis.
  • Fig. S7. Representation of amino acid losses in genome-guided MALDI-TOF/ TOF analysis for Sh-lantibiotic-β.
  • Fig. S8. Skin isolate strains of CoNS exert selective antimicrobial activity against S. aureus.
  • Fig. S9. Detection of Sh-lantibiotic-α peptide by Western blotting from extracts of skin swabs taken from two non-AD subjects and two AD subjects.
  • Fig. S10. List of hypothetical antimicrobial genes identified in anti–S. aureus CoNS clones used for AMT.
  • Fig. S11. List of previously known lantibiotic genes identified in antimicrobial S. epidermidis clones isolated from non-AD skin.
  • Fig. S12. Study design of AMT therapy for patients with AD.
  • Fig. S13. Effect of transplantation of antimicrobial CoNS or vehicle on the survival of S. aureus on the skin of five subjects with AD.
  • Table S1. Clinical characteristics of AD and non-AD subjects.
  • Table S2. Proportion of CoNS species identified with antimicrobial and nonantimicrobial activity.
  • Table S3. Clinical characteristics of AD subjects receiving AMT.
  • Table S4. Sequences of PCR primers.
  • References (4563)

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Other Supplementary Material for this manuscript includes the following:

  • Table S5. Primary data (provided as an Excel file).