Supplementary Materials

Supplementary Material for:

Two-step enhanced cancer immunotherapy with engineered Salmonella typhimurium secreting heterologous flagellin

Jin Hai Zheng, Vu H. Nguyen, Sheng-Nan Jiang, Seung-Hwan Park, Wenzhi Tan, Seol Hee Hong, Myung Geun Shin, Ik-Joo Chung, Yeongjin Hong, Hee-Seung Bom, Hyon E. Choy, Shee Eun Lee, Joon Haeng Rhee,* Jung-Joon Min*

*Corresponding author. Email: jjmin{at}jnu.ac.kr (J.-J.M.); jhrhee{at}chonnam.ac.kr (J.H.R.)

Published 8 February 2017, Sci. Transl. Med. 9, eaak9537 (2017)
DOI: 10.1126/scitranslmed.aak9537

This PDF file includes:

  • Materials and Methods
  • Fig. S1. NF-κB activation by LPS and FlaB in cancer cells and peritoneal macrophages in vitro.
  • Fig. S2. Luciferase assay in HCT116 cancer cells.
  • Fig. S3. Spleen weight after Salmonella treatment.
  • Fig. S4. Analysis of cell populations in the spleen after Salmonella treatment.
  • Fig. S5. Noninvasive monitoring of bacterial distribution in vivo.
  • Fig. S6. Distribution of bacteria in MC38 tumor–bearing mice.
  • Fig. S7. Detection of bacteria and FlaB in liver and tumor tissues.
  • Fig. S8. Systemic toxicity of FlaB-expressing bacteria.
  • Fig. S9. Photographs of mice treated with FlaB-secreting bacteria.
  • Fig. S10. Antitumor effect in a B16F10 melanoma model.
  • Fig. S11. Tumor growth in WT and knockout mice.
  • Fig. S12. Effect of bacterial treatments on tumor growth in TLR4 knockout mice.
  • Fig. S13. Cell infiltration in WT and knockout mice after Salmonella treatment.
  • Fig. S14. Macrophage polarization after treatment with FlaB-secreting bacteria assessed by quadruple staining.
  • Fig. S15. Detection of tumor-suppressive cytokines in tumor tissues.
  • Table S1. Bacterial strains and plasmids used in the study.
  • Table S2. Antibodies used in the study.

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