Supplementary Materials

Supplementary Material for:

IgA-coated E. coli enriched in Crohn's disease spondyloarthritis promote TH17-dependent inflammation

Monica Viladomiu, Charles Kivolowitz, Ahmed Abdulhamid, Belgin Dogan, Daniel Victorio, Jim G. Castellanos, Viola Woo, Fei Teng, Nhan L. Tran, Andrew Sczesnak, Christina Chai, Myunghoo Kim, Gretchen E. Diehl, Nadim J. Ajami, Joseph F. Petrosino, Xi K. Zhou, Sergio Schwartzman, Lisa A. Mandl, Meira Abramowitz, Vinita Jacob, Brian Bosworth, Adam Steinlauf, Ellen J. Scherl, Hsin-Jung Joyce Wu, Kenneth W. Simpson, Randy S. Longman*

*Corresponding author. Email: ral2006{at}med.cornell.edu

Published 8 February 2017, Sci. Transl. Med. 9, eaaf9655 (2017)
DOI: 10.1126/scitranslmed.aaf9655

This PDF file includes:

  • Materials and Methods
  • Fig. S1. α- and β-diversity metrics of 16S rRNA compositional data.
  • Fig. S2. α- and β-diversity metrics stratified by immunomodulator exposure.
  • Fig. S3. 16S rRNA IgA-seq sample rarefaction.
  • Fig. S4. Relative abundance by phylum, family, and genus in the presort fraction.
  • Fig. S5. α- and β-diversity of 16S rRNA IgA-seq data.
  • Fig. S6. Relative abundance by phylum and family in the sorted IgA+ and IgAfractions.
  • Fig. S7. CD-SpA–derived, IgA-coated E. coli isolates are AIEC.
  • Fig. S8. Circular genome map of CD-SpA–derived E. coli isolate 2A.
  • Fig. S9. Colonization of germ-free C57BL/6 mice with CD-SpA E. coli 2A.
  • Fig. S10. CD-SpA E. coli 2A induces TH17 mucosal immunity.
  • Fig. S11. CD-SpA E. coli 2A induces ILC3 mucosal immunity.
  • Fig. S12. Characterization of dendritic cells, CD8+ T cells, and Tfh.
  • Fig. S13. Assessment of colonization.
  • Fig. S14. CD-SpA AIEC 2A protects WT mice against DSS.
  • Table S1. Demographics, clinical parameters, and disease activity indexes of study subjects.
  • Table S2. Montreal classification of CD cohort.
  • Table S3. AIEC marker genes in reference strains and clonal isolates from CD and CD-SpA patients.
  • References (6370)

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Other Supplementary Material for this manuscript includes the following: