Supplementary Materials

Supplementary Material for:

Thioredoxin reverses age-related hypertension by chronically improving vascular redox and restoring eNOS function

Rob H. P. Hilgers, Venkatesh Kundumani-Sridharan, Jaganathan Subramani, Leon C. Chen, Luis G. Cuello, Nancy J. Rusch, Kumuda C. Das*

*Corresponding author. Email: kumuda.das2{at}uthct.edu

Published 8 February 2017, Sci. Transl. Med. 9, eaaf6094 (2017)
DOI: 10.1126/scitranslmed.aaf6094

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Aging oxidizes vascular TRX in NT and dnTrx-Tg mice but not in Trx-Tg mice.
  • Fig. S2. MAP of aged Trx-Tg mice is lower than that of aged WT mice.
  • Fig. S3. Peak systolic pressure is decreased in aged WT mice injected with rhTRX relative to aged WT mice.
  • Fig. S4. Losartan acutely reduces MAP but does not chronically maintain MAP.
  • Fig. S5. Depletion of TRX by siRNA decreases SMA relaxation.
  • Fig. S6. TRX does not preserve total BH4 concentration, and sepiapterin does not reverse endothelial dysfunction.
  • Fig. S7. DTT, but not TEMPOL, restores vascular relaxation in SMA from aged NT mice.
  • Fig. S8. TRX prevents eNOS S-glutathionylation and maintains NOX4 protein concentrations.
  • Table S1. Summary of all P values for all figures presented.

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