Supplementary Materials

Supplementary Material for:

Fingolimod treatment abrogates chikungunya virus–induced arthralgia

Teck-Hui Teo, Yi-Hao Chan, Wendy W. L. Lee, Fok-Moon Lum, Siti Naqiah Amrun, Zhisheng Her, Ravisankar Rajarethinam, Andres Merits, Olaf Rötzschke, Laurent Rénia,* Lisa F. P. Ng*

*Corresponding author. Email: isa_ng{at}immunol.a-star.edu.sg (L.F.P.N.); renia_laurent{at}immunol.a-star.edu.sg (L.R.)

Published 1 February 2017, Sci. Transl. Med. 9, eaal1333 (2017)
DOI: 10.1126/scitranslmed.aal1333

This PDF file includes:

  • Methods
  • Fig. S1. Restoration of total and CHIKV-specific CD4+ T cells in TCR−/− adoptive transfer model.
  • Fig. S2. Joint footpad with an absence of musculoskeletal pathology in WT mock and TCR−/− mock + CD4+ T cell transfer mice.
  • Fig. S3. Histopathological evaluation of bone, cartilages, and synovial membrane in CHIKV-infected mice.
  • Fig. S4. CHIKV epitopes induce IFNγ in splenocytes of acute CHIKV infection ex vivo.
  • Fig. S5. Primary CD4+ T cell line against dominant CHIKV CD4+ T cell epitopes respond to antigen stimulation in a TH1-biased manner.
  • Fig. S6. Expansion of primary nsP1-P4-2 and E2EP3 lines in TCR−/− recipient mice after CHIKV infection.
  • Fig. S7. Epitope-specific CD4+ T cell line transfer into virus-infected TCR−/− mice recapitulates inflammation with no effect on viremia.
  • Fig. S8. Rapamycin and CsA treatments aggravate joint pathology during CHIKV infection.
  • Fig. S9. Histopathological evaluation of bone, cartilages, and synovial membrane of fingolimod-treated CHIKV-infected mice.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Data sets of all main figures.
  • Table S2 (Microsoft Excel format). Data sets of all supplementary figures.

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