Supplementary Materials

Supplementary Material for:

Abnormal neurogenesis and cortical growth in congenital heart disease

Paul D. Morton, Ludmila Korotcova, Bobbi K. Lewis, Shivaprasad Bhuvanendran, Shruti D. Ramachandra, David Zurakowski, Jiangyang Zhang, Susumu Mori, Joseph A. Frank, Richard A. Jonas,* Vittorio Gallo,* Nobuyuki Ishibashi*

*Corresponding author. Email: vgallo{at}childrensnational.org (V.G.); nishibas{at}childrensnational.org (N.I.); rjonas{at}childrensnational.org (R.A.J.)

Published 25 January 2017, Sci. Transl. Med. 9, eaah7029 (2017)
DOI: 10.1126/scitranslmed.aah7029

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Structural similarities are observed between the neonatal piglet and fetal and infant human SVZ.
  • Fig. S2. oRG populate the postnatal piglet SVZ.
  • Fig. S3. Regional and temporal differences in NSPC numbers are observed in the postnatal piglet SVZ.
  • Fig. S4. Neurospheres generated from the piglet SVZ display regional and temporal heterogeneity.
  • Fig. S5. Migrating neuroblasts are abundant in the developing piglet brain.
  • Fig. S6. Immature neurons are seen in the piglet brain extending laterally from the anterior SVZ to the cortex.
  • Fig. S7. SPIO-impregnated cells are distributed throughout the piglet brain.
  • Fig. S8. T2* scans illustrate the distribution of SPIO nanoparticles compared with standard T2 MRI in the piglet brain.
  • Fig. S9. Distribution patterns of interneurons vary by subtype within the piglet prefrontal cortex.
  • Fig. S10. The abundance of neuroblasts within the A-DL–SVZ declines with age.
  • Fig. S11. Chronic hypoxia reduces the size of the piglet SVZ independent of cell death.
  • Fig. S12. Chronic hypoxia reduces NSPC numbers and activity in the piglet AE-SVZ.
  • Fig. S13. Chronic hypoxia reduces the number of immature neurons in layers II/III of the piglet frontal cortex.
  • Table S1. Minimal colocalization was observed between focally labeled cells and birth dating transcription factors indicative of ganglionic eminence origin in p14 piglets.
  • Table S2. Quantification and statistical analysis reveals the distribution and cell fate of SPIO-impregnated cells in the piglet cortex, 4 wpi.
  • Table S3. Human brain tissue was obtained from two cohorts of infants of similar ages.
  • Table S4. Regional assessment of excitatory and inhibitory neuron populations shows specific alterations in cell numbers throughout the piglet cortex after hypoxic exposure, p14.
  • Table S5. Group analysis of excitatory and inhibitory neurons throughout the piglet cortex shows specific regional differences after hypoxic exposure, p14.
  • Table S6. P values for all significant findings not listed in the main text.
  • References (6267)

[Download PDF]